| TR短肽修饰的DSPE-PEG胶束对CD133高表达胶质瘤肿瘤干细胞体外靶向性评价(英文) |
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| 引用本文： | 王景达,何冰,代文兵,王学清,王坚成,张烜,张强. TR短肽修饰的DSPE-PEG胶束对CD133高表达胶质瘤肿瘤干细胞体外靶向性评价(英文)[J]. 中国药学, 2015, 24(1): 34-39. DOI: 10.5246/jcps.2015.01.004 |
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| 作者姓名： | 王景达 何冰 代文兵 王学清 王坚成 张烜 张强 |
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| 作者单位： | 北京大学医学部天然药物及仿生药物国家重点实验室;药学院药剂学系 |
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| 基金项目： | The Key Program of National Science Foundation(Grant No.81130059);the National Basic Research Program of China(Grant No.973 program,2013CB932501) |
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| 摘 要： | 肿瘤干细胞已经成为药物递送的一个新靶点。肿瘤干细胞表面表达特异性标志物,其中CD133在胶质瘤肿瘤干细胞中高表达。因此,我们通过将特异性结合CD133的TR短肽修饰到药物递送系统上,来达到靶向递送药物的胶质瘤干细胞的目的。首先,我们将TR短肽与DSPE-PEG连接,构建包载香豆素-6的主被动DSPE-PEG胶束。之后,我们通过荧光激发免疫细胞分选法和肿瘤球培养法分离胶质瘤肿瘤干细胞。通过共聚焦显微镜和流式细胞仪检测发现,肿瘤干细胞对T R修饰的胶束摄取增多,证明TR修饰胶束具有体外靶向性。由此表明,TR修饰胶束有可能提供了针对CD133+肿瘤干细胞的新的治疗策略。
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| 关 键 词： | 肿瘤干细胞 CD133 TR短肽 胶束 肿瘤球 荧光激发免疫细胞分选 |
| 收稿时间： | 2014-07-19 |
Targeting glioblastoma cancer stem cell marker CD133 by heptapeptide-modified DSPE-PEG micelles |
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| Jingda Wang;Bing He;Wenbing Dai;Xueqing Wang;Jiancheng Wang;Xuan Zhang;Qiang Zhang. Targeting glioblastoma cancer stem cell marker CD133 by heptapeptide-modified DSPE-PEG micelles[J]. Journal of Chinese Pharmaceutical Sciences, 2015, 24(1): 34-39. DOI: 10.5246/jcps.2015.01.004 |
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| Authors: | Jingda Wang Bing He Wenbing Dai Xueqing Wang Jiancheng Wang Xuan Zhang Qiang Zhang |
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| Affiliation: | Jingda Wang;Bing He;Wenbing Dai;Xueqing Wang;Jiancheng Wang;Xuan Zhang;Qiang Zhang;State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center; |
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| Abstract: | Cancer stem cells have become the new target of chemotherapy with specific cell marker. CD133 is shown to be highly expressed in glioma cancer stem cells. In this study, a drug delivery system is designed to target CD133 by a seven amino acid peptide (TR peptide), which is capable to specifically bind to CD133. First, TR was conjugated to DSPE-PEG and coumarin-6 was loaded into the DSPE-PEG micelles. Then fluorescence-activated cell sorting (FACS) and tumorsphere culture were conductedto isolate cancer stem cells in C6 cells. The enhanced uptake of micelles was observed by confocal microscopy and flow cytometry,suggesting that TR peptide-modified micelles may exhibit better anti-cancer efficacy by targeting CD133+ glioma stem cells. In conclusion, TR peptide-modified micelles may provide new strategy to achieve enhanced specificity to CD133+ glioma stem cells. |
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| Keywords: | Cancer stem cell CD133 TR peptide Micelle Tumorsphere FACS |
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