| 和颜坤泰胶囊对卵巢去势大鼠子宫形态及细胞凋亡的影响 |
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| 引用本文： | 黄春丽,张德芹,张青霞,文静. 和颜坤泰胶囊对卵巢去势大鼠子宫形态及细胞凋亡的影响[J]. 天津中医药大学学报, 2016, 33(11): 675-681 |
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| 作者姓名： | 黄春丽 张德芹 张青霞 文静 |
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| 作者单位： | 天津中医药大学中医药研究院, 天津 300193,天津中医药大学中医药研究院, 天津 300193,天津中医药大学中医药研究院, 天津 300193,天津中医药大学中医药研究院, 天津 300193 |
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| 基金项目： | 国家重大新药创制科技重大专项（2012ZX09304007）。 |
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| 摘 要： | [目的]研究和颜坤泰胶囊对卵巢去势模型大鼠子宫形态及细胞凋亡的影响。[方法]以雌性去势大鼠为动物模型，分为空白对照组，假手术组，模型组，补佳乐组，莉芙敏组，和颜坤泰胶囊8、4、2 g生药/kg剂量组。给药8周后，观察药物对子宫病理学的影响，检测子宫细胞凋亡率及Bcl-2 mRNA、Bax mRNA和蛋白的表达。[结果]和颜坤泰胶囊可增加去势大鼠子宫横截面及壁厚，肌层面积及厚度，内膜面积及厚度，上皮厚度和腺体数（P<0.01，P<0.05）；能降低去势大鼠子宫细胞的凋亡率（P<0.01，P<0.05），升高子宫Bcl-2 mRNA和蛋白的表达（P<0.01，P<0.05），降低BaxmRNA和蛋白的表达（P<0.01，P<0.05）。[结论]和颜坤泰胶囊有一定的子宫营养作用，可通过调控Bcl-2和Bax基因与蛋白的表达来抑制子宫细胞的凋亡。
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| 关 键 词： | 和颜坤泰胶囊 子宫形态学 细胞凋亡 Bcl-2 mRNA和蛋白 Bax mRNA和蛋白 |
| 收稿时间： | 2016-04-21 |
Anti-apoptotic and uterotrophic effects of Heyan Kuntai capsules in uterus of ovariectomized rats |
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| HUANG Chun-li,ZHANG De-qin,ZHANG Qing-xia and WEN Jing. Anti-apoptotic and uterotrophic effects of Heyan Kuntai capsules in uterus of ovariectomized rats[J]. Journal of Tianjin University of Traditonal Chinese Medicine, 2016, 33(11): 675-681 |
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| Authors: | HUANG Chun-li ZHANG De-qin ZHANG Qing-xia WEN Jing |
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| Affiliation: | Traditional Chinese Medicine Research Institute of Tianjin University of Triditional Chinese Medicine, Tianjin 300193, China,Traditional Chinese Medicine Research Institute of Tianjin University of Triditional Chinese Medicine, Tianjin 300193, China,Traditional Chinese Medicine Research Institute of Tianjin University of Triditional Chinese Medicine, Tianjin 300193, China and Traditional Chinese Medicine Research Institute of Tianjin University of Triditional Chinese Medicine, Tianjin 300193, China |
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| Abstract: | [Objective] To investigate the effects of Heyan Kuntai capsules on uterus morphology and apoptosis in ovariectomized rats.[Methods] Female SD rats underwent ovariectomize surgery and were divided into model group, progynova group, Remifemin group and Heyan Kuntai capsule of high, medium and low dose groups (8, 4 and 2 g crude drug/kg respectively). The drugs were administered for 8 weeks. This article observed the impacts of drugs on uterus pathology, staining and apoptosis, detected the expression levels of Bcl-2 and Bax mRNAs and proteins.[Results] It was obtained significantly decreased epithelial thickness and reduced myometrium and endometrium cross-sectional area and wall thickness in the model group compared with the control and Heyan Kuntai capsule treated groups(P<0.01 or P<0.05). It was also decreased Bcl-2 and increased Bax mRNAs and proteins expressions(P<0.01 or P<0.05) in the model group. Further more, the model group showed increased apoptosis rate(P<0.01 or P<0.05) and reduced number of glands(P<0.01 or P<0.05) in the uterus.[Conclusion] Heyan Kuntai capsules elicits uterotrophic effect and attenuates apoptosis in the uterus cells by regulating mRNAs and protein expressions of Bcl-2 and Bax. |
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| Keywords: | Heyan Kuntai capsule uterus morphology apoptosis Bcl-2 mRNA and protein Bax mRNA and protein |
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