| HCV C基因腺病毒表达载体骨架质粒pAd.HCV-C的构建、鉴定及表达 |
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| 引用本文： | 郝春秋,周永兴,冯志华,李谨革,贾战生,王平忠. HCV C基因腺病毒表达载体骨架质粒pAd.HCV-C的构建、鉴定及表达[J]. 世界华人消化杂志, 2001, 9(6): 635-639 |
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| 作者姓名： | 郝春秋 周永兴 冯志华 李谨革 贾战生 王平忠 |
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| 作者单位： | 中国人民解放军第四军医大学唐都医院全军传染病防治中心,陕西省,西安市,710038 |
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| 基金项目： | 国家自然科学基金课题,No.39800122. |
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| 摘 要： |
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| 关 键 词： | C型肝炎样病毒属/遗传学 基因,病毒 腺病毒科/遗传学 遗传载体 质粒 重组,遗传 |
| 修稿时间： | 2001-04-05 |
Construction, identification and expression of framework plasmid pAd. HCV-C of adenovirus expression vector of HCV C |
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| Chun Qiu Hao,Yong Xing Zhou,Zhi Hua Feng,Jin Ge Li,Zhan Sheng Jia,Ping Zhong Wang. Construction, identification and expression of framework plasmid pAd. HCV-C of adenovirus expression vector of HCV C[J]. World Chinese Journal of Digestology, 2001, 9(6): 635-639 |
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| Authors: | Chun Qiu Hao Yong Xing Zhou Zhi Hua Feng Jin Ge Li Zhan Sheng Jia Ping Zhong Wang |
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| Abstract: | AIM To construct a recombinant framework plasmid of adenovirus expression vector which can express HCV core gene. This should be useful to pack adenovirus expression vector which can express HCV core gene effectively. METHODS The HCV core gene derived from the plasmid pBRTM/HCV 1-3011 by using polymerase chain reaction (PCR) was inserted into the backward position of cytomegalovirus (CMV) immediate early promotor element of framework plasmid (pAd. CMV-Link. 1) of adenovirus expression vector, then the recombinant plasmid pAd. HCV-C was obtained. The pad. HCV-C was identified by endonuclease, PCR and sequencing. HCV core gene was expressed transiently with Lipofectamine coated in human hepatoblastoma 7721 cells which examined by indirect immunofluorescence. RESULTS Insert DNA of pAd. HCV-C was confirmed HCV core gene by endonuclease, PCR and sequencing, pAd. HCV-C can express HCV core gene transiently in 7721 cells which confirmed by indirect immunofluorescence. CONCLUSION Recombinant framework plasmid of adenovirus expression vector pAd. HCV-C can express HCV core gene transiently in 7721 cells. This should be useful to pack adenovirus expression vector which can express HCV core gene. |
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| Keywords: | hepatitis C-like viruses/genetics genes viral adenoviridae/genetics genetic vectors plasmids recombination genetic |
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