Insulin autoantibodies in non-obese diabetic (NOD) mice. |
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| Authors: | C Michel C Boitard J F Bach |
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| Affiliation: | Service d''Immunologie Clinique, INSERM U 25, CNRS UA 122 Hôpital Necker, Paris, France. |
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| Abstract: | Anti-insulin autoantibodies were detected in NOD mice using ELISA. The antibodies were detected as early as at 5 weeks of age, long before onset of clinically overt diabetes, especially in diabetes-prone female mice. The anti-insulin specificity was verified by passage on affinity chromatography insulin columns and demonstration that the anti-insulin activity was located on the F(ab')2 region of the immunoglobulins. The presence of anti-insulin antibodies in prediabetic NOD mice provides a unique possibility for studying their significance and their eventual pathogenic role in the development of insulin-dependent diabetes. |
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