A New Relaxed Mutant of Escherichia coli with an Altered 50S Ribosomal Subunit |
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| Authors: | J. D. Friesen N. P. Fiil J. M. Parker William A. Haseltine |
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| Affiliation: | *Department of Biology, York University, 4700 Keele Street, Downsview, Ontario M3J 1P3, Canada;†University Institute of Microbiology, Øster Farimagsgade 2A, Copenhagen K, Denmark;‡Massachusetts Institute of Technology, Cambridge, Mass. 02139 |
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| Abstract: | A new relaxed mutant called rel C has been isolated from a rel A(+)/rel A(+) partial diploid strain. The rel C mutant is unable to synthesize ppGpp or pppGpp in vivo in response to amino acid starvation or in vitro, but can synthesize these nucleotides in a shift-down. Rel C maps near rif. Studies in vitro demonstrate the lesion to be probably in one of the 50S ribosomal proteins that can be removed by 1.0 M LiCl. |
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