| 18F-FDG micro-PET/CT在活体大鼠脑出血模型中的诊断价值 |
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| 引用本文： | 杨凡慧,杨朝鲜,张春银. 18F-FDG micro-PET/CT在活体大鼠脑出血模型中的诊断价值[J]. 国际放射医学核医学杂志, 2021, 45(4): 231-236. DOI: 10.3760/cma.j.cn121381-202003023-00045 |
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| 作者姓名： | 杨凡慧 杨朝鲜 张春银 |
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| 作者单位： | 川北医学院附属医院核医学科，南充 637000;西南医科大学附属医院核医学与分子影像四川省重点实验室，泸州 646000;西南医科大学神经生物学研究室，泸州 646000;西南医科大学附属医院核医学科，泸州 646000 |
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| 摘 要： | 目的:评价 18F-氟脱氧葡萄糖（FDG） micro-PET/CT对活体大鼠脑出血（ICH）模型的诊断价值。 方法:采用简单随机抽样法选取32只健康雄性成年SD大鼠，其中28只在大鼠大脑右侧基底节区注射0.125 U/μL胶原酶Ⅳ构建大鼠ICH模型（ICH模型组），其余4只以0.9%生理盐水代替...
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| 关 键 词： | 脑出血 正电子发射断层显像术 体层摄影术，X线计算机 氟脱氧葡萄糖F18 大鼠 模型，动物 |
| 收稿时间： | 2020-03-16 |
Diagnostic value of 18F-FDG micro-PET/CT in rat models of intracerebral hemorrhage in vivo |
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| Fanhui Yang,Chaoxian Yang,Chunyin Zhang. Diagnostic value of 18F-FDG micro-PET/CT in rat models of intracerebral hemorrhage in vivo[J]. International Journal of Radiation Medicine and Nuclear Medicine, 2021, 45(4): 231-236. DOI: 10.3760/cma.j.cn121381-202003023-00045 |
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| Authors: | Fanhui Yang Chaoxian Yang Chunyin Zhang |
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| Affiliation: | 1.Department of Nuclear Medicine, the Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, China |
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| Abstract: | Objective To assess the diagnostic value of 18F-fluorodeoxyglucose (FDG) micro-PET/CT in evaluating an intracerebral hemorrhage (ICH) model in rats in vivo. Methods A simple random sampling method was used to select 32 healthy male adult SD rats, 4 of which were placed in the sham operation group and 28 rats were injected with 0.125 U/μL collagenase Ⅳ into the right basal ganglia to induce ICH (ICH model group). In the sham operation group, 0.9% saline was used instead of collagenase Ⅳ to make the sham model. The ICH model group was divided into seven groups by simple random sampling according to the time after ICH, which were 6, 24, 48 h and 3, 5, 7, 14 d (4 rats in each group). The sham operation and seven ICH model groups (6, 24, 48 h and 3, 5, 7, 14 d) underwent the neurological impairment scoring and 18F-FDG micro-PET/CT imaging. The hematoma volume at each time point in the ICH model group was calculated according to 18F-FDG micro-PET/CT imaging to delineate the region of interest (ROI) and Tada's formula. After imaging, the head was decapitated and the brain was obtained for hematoma observation and histopathological examination. The hematoma volume obtained by the two methods at the same time was compared by paired t-test and evaluated by Pearson correlation analysis. Results In the sham operation group: the neurological impairment scores were zero; and micro-PET/CT clearly showed homogeneous 18F-FDG uptake in the brain tissue. The ICH model group: the neurological impairment scores were (2.21±0.30), (3.51±0.66), (2.83±0.20), (2.12±0.50), (1.44±0.37), (1.02±0.25) and (0.51±0.12) at 6, 24, 48 h and 3, 5, 7, 14 d after ICH, respectively. At each time point, the 18F-FDG uptake decreased or became defective in the right basal ganglia of the rat brain. The cerebral hematoma volumes evaluated by 18F-FDG micro-PET/CT were (24.05±3.00), (27.19±1.25), (25.58±1.57), (21.94±0.98), (19.88±1.53), (18.35±2.11) and (16.29±1.53) mm3, respectively. The cerebral hematoma volumes evaluated by Tada's formula were (23.17±1.93), (26.09±1.35), (24.64±1.95), (21.31±1.32), (19.07±1.64), (17.29±1.38), and (15.63±1.98) mm3, respectively. No significant difference in the hematoma volume was found between two methods at each time point in the ICH model group (t=1.18?3.06, all P>0.05). The cerebral hematoma volume obtained by the two methods was significantly positively correlated (r=0.99, P<0.001). After the dissection of the ICH model group, irregular hematoma formation was seen in the right basal ganglia of the brain tissue, which was opposite to the radioactive sparse and defective areas shown by 18F-FDG micro-PET/CT. Conclusion 18F-FDG micro-PET/CT can accurately display the position, shape and size of the hematoma after ICH, and thus can be to verify the success of the rat ICH model in vivo. |
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