| Circ_0007142吸附miR-647调控CCR8基因促进胃癌细胞的上皮-间质转化和侵袭 |
| |
| 引用本文： | 张学成,关晓辉. Circ_0007142吸附miR-647调控CCR8基因促进胃癌细胞的上皮-间质转化和侵袭[J]. 中国癌症杂志, 2021, 31(8): 714-724. DOI: 10.19401/j.cnki.1007-3639.2021.08.004 |
| |
| 作者姓名： | 张学成 关晓辉 |
| |
| 作者单位： | 1. 吉林市中心医院消化科，吉林 吉林 132000 ；2. 北华大学附属医院消化内科，吉林 吉林 132000 |
| |
| 基金项目： | 吉林省卫生厅科技计划项目（2013S004）。 |
| |
| 摘 要： | 背景与目的:胃癌是常见的消化道恶性肿瘤,circ_0007142被证实为结直肠癌的致癌因子,能促进结直肠癌的进展.探究circ_0007142吸附miR-647调控CCR8基因对胃癌细胞上皮-间质转化(epithelial-mesenchymal transition,EMT)和侵袭的影响.方法:采用实时荧光定量聚合酶...
|
| 关 键 词： | Circ_0007142 miR-647 CCR8 胃癌 上皮-间质转化 侵袭 |
Circ_0007142 accelerates epithelial-mesenchymal transition and invasion of gastric cancer cells through sponging miR-647 and regulating CCR8 gene |
| |
| ZHANG Xuecheng,GUAN Xiaohui. Circ_0007142 accelerates epithelial-mesenchymal transition and invasion of gastric cancer cells through sponging miR-647 and regulating CCR8 gene[J]. China Oncology, 2021, 31(8): 714-724. DOI: 10.19401/j.cnki.1007-3639.2021.08.004 |
| |
| Authors: | ZHANG Xuecheng GUAN Xiaohui |
| |
| Affiliation: | 1. Department of Gastroenterology, Beihua University, Jilin Central Hospital, Jilin 132000, Jilin Province, China; 2. Department of Gastroenterology, Affiliated Hospital of Beihua University, Jilin 132000, Jilin Province, China; |
| |
| Abstract: | Background and purpose: Gastric cancer is the most common malignant tumor of digestive tract. Circ_0007142 has been proved to be a carcinogenic factor of colorectal cancer and can promote the progression of colorectal cancer. This study aimed to explore the effects of circ_0007142 on epithelial-mesenchymal transition (EMT) and invasion of gastric cancer cells via absorbing miR-647 and then regulating CCR8 gene. Methods: Real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR) was used to detect the expressions of circ_0007142, miR-647 and CCR8 in gastric cancer tissues and cells. Fluorescence in situ hybridization (FISH) experiment was adopted to determine the subcellular localization of circ_0007142. Dual luciferase reporter experiment and RNA immunoprecipitation (RIP) assay were used to confirm the targeting relationship of circ_0007142 and miR-647 as well as miR-647 and CCR8. Transwell assay, clone formation assay and Western blot were used to test the cell invasion ability, clonality and EMT respectively. Tumor xenograft in BALB/c nude mice was performed to detect tumorigenicity of circ_0007142 in vivo. Results: Overexpressions of circ_0007142 and CCR8 and downregulation of miR-647 were detected in gastric cancer tissues and cells. Circ_0007142 acted as a molecular sponge to inhibit the expression of miR-647, at the same time, miR-647 inhibited theexpression of CCR8 by binding with the 3'-UTR of CCR8 mRNA. Knockdown of circ_0007142 or overexpression of miR-647 inhibited the invasion, colony formation and EMT of gastric cancer cells. However, the effects of circ_0007142 inhibition or miR-647 overexpression on gastric cancer cells were partially reversed by miR-647 inhibitor or CCR8 overexpression (all P<0.05). Moreover, knockdown of circ_0007142 in gastric cancer cells inhibited the tumor growth in vivo. Conclusion: Circ_0007142 upregulates the expression of CCR8 via sponging miR-647, which subsequently accelerates the EMT and invasion of gastric cancer cells and promotes the progression of gastric cancer. |
| |
| Keywords: | Circ_0007142 miR-647 CCR8 Gastric cancer Epithelial-mesenchymal transition Invasion |
|
| 点击此处可从《中国癌症杂志》浏览原始摘要信息 |
|
点击此处可从《中国癌症杂志》下载全文 |
|
