| 洛美利嗪对大鼠脑微血管内皮细胞上P-糖蛋白功能的影响 |
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| 引用本文： | 洛美利嗪对大鼠脑微血管内皮细胞上P-糖蛋白功能的影响[J]. 中国药科大学学报, 2003, (4): 62-65. |
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| 作者姓名： | 吴玉林 祝浩杰 郑莉 刘国卿 |
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| 作者单位： | 1. 中国药科大学生理学教研室,南京,210009
2. 中国药科大学药理学教研室,南京,210009 |
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| 摘 要： | 目的：研究洛美利嗪对大鼠脑微血管内皮细胞上P-糖蛋白功能的影响。方法：使用荧光分光光度计和流式细胞术分析大鼠脑微血管内皮细胞内P-糖蛋白底物-罗丹明123的荧光强度。结果：环孢素A和洛美利嗪与大鼠脑微血管内皮细胞孵育后能明显提高胞内罗丹明123的荧光强度。人脐静脉内皮细胞内荧光强度则不受环孢素A和洛美利嗪的影响。结论：洛美利嗪能显著地抑制大鼠脑微血管内皮细胞上P-糖蛋白的活性，提高P-糖蛋白底物的胞内浓度。
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| 关 键 词： | P-糖蛋白 罗丹明123 大鼠脑微血管内皮细胞 流式细胞术 洛美利嗪 |
| 文章编号： | 1000-5048(2003)04-0352-04 |
Effect of Lomerizine on the Activity of P-glycoprotein in Primary Cultured Rat Brain Microvessel Endothelial Cells |
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| Effect of Lomerizine on the Activity of P-glycoprotein in Primary Cultured Rat Brain Microvessel Endothelial Cells[J]. Journal of China Pharmaceutical University, 2003, (4): 62-65. |
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| Authors: | WU Yu-Ling ZHU Hao-Jie ZHENG Li LIU Guo-Qing |
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| Affiliation: | WU Yu-Ling 1,ZHU Hao-Jie 2,ZHENG Li 2,LIU Guo-Qing 2 1Department of Pharmacology, 2 Department of Physiology,China Pharmaceutical University,Nanjing 210009,China |
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| Abstract: | AIM:To study the effect of lomerizine on the activity of P-glycorprotein(P-gp) in primary cultured rat brain microvessel endothelial cells. METHOD: Fluorescence of Rhodamine123(Rh123) in RBMECs was measured by fluorescence spectrophotometry and flow cytometry. RESULT:Cellular fluorescence of Rh123 in RBMECs increased significantly after incubation with CsA and lomerizine. As a negative control, CsA and lomerizine have no effect on accumulation of Rhodamine123 in HUVECs. CONCLUTION:Lomerizine has a concentration-dependent inhibitory effect on the activity of P-glycoprotein in RBMECs and results in increases in the accumulation of P-glycoprotein substrates. |
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| Keywords: | P-glycoprotein Rhodamine123 Rat Brain microvessel endothelial cells Flow cytometry Lomerizine |
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