| IFN-γ活化小鼠腹腔巨噬细胞抗弓形虫作用的剂量相关性及其与TNF-α的协同作用 |
| |
| 引用本文： | 张爱民,杨惠珍,杨杨,钱宗立. IFN-γ活化小鼠腹腔巨噬细胞抗弓形虫作用的剂量相关性及其与TNF-α的协同作用[J]. 中国寄生虫学与寄生虫病杂志, 1998, 16(6): 436-440 |
| |
| 作者姓名： | 张爱民 杨惠珍 杨杨 钱宗立 |
| |
| 作者单位： | 上海市免疫研究所寄生虫学研究室 |
| |
| 摘 要： | 目的：揭示IFN－γ抗弓形虫作用的剂量相关性及TNF－α对IFN－γ是否存在协同作用。方法：本实验体外以不同剂量IFN－γ单独或与TNF－α联合刺激昆明鼠腹腔巨噬细胞，观察其对入侵的RH株速殖子的作用及培养上清中NO的水平。结果：随着IFN－γ剂量的增大，巨噬细胞抗虫作用增强，培养上清中NO水平增高；虫体入侵后24hNO水平与细胞内虫体数呈显著负相关。结论：IFN－γ可激活巨噬细胞抑制和杀灭入侵的弓形虫，并与剂量有关，TNF－α对此有协同作用，NO是抗虫作用中重要的效应因子。
|
| 关 键 词： | 弓形虫 γ－干扰素 肿瘤坏死因子－α 巨噬细胞 氧化氮 |
Correlation between the dose and the anti-Toxoplasma effect of activated mouse macrophages induced by IFN-gamma and the synergism between IFN-gamma and TNF-alpha] |
| |
| Zhang Aimin,Yang Huizhen,Yang Yang,Qian Zhongli. Correlation between the dose and the anti-Toxoplasma effect of activated mouse macrophages induced by IFN-gamma and the synergism between IFN-gamma and TNF-alpha][J]. Chinese Journal of Parasitology and Parasitic Diseases, 1998, 16(6): 436-440 |
| |
| Authors: | Zhang Aimin Yang Huizhen Yang Yang Qian Zhongli |
| |
| Affiliation: | Laboratory of Parasitology, Shanghai Institute of Immunology, Shanghai 200025. |
| |
| Abstract: | AIM: To find out the dose dependence of the anti-Toxoplasma effect induced by IFN-gamma and to determine the possible synergistic activity between TNF-alpha and IFN-gamma. METHODS: The in vitro effect of cultivated mouse peritoneal macrophages activated by IFN-gamma alone or IFN-gamma combined with different doses of TNF-alpha on the intracellular tachyzoites of RH strains and the nitric oxide (NO) level in the culture medium supernatant were simultaneously determined. RESULTS: With the increase in the dose of IFN-gamma, the anti-Toxoplasma effect was augmented and the NO level was enhanced. At 24 hours after tachyzoite invasion, a significant reversed correlation was demonstrated between the NO level and the number of intracellular parasites. CONCLUSION: The anti-Toxoplasma effect of macrophages activated by IFN-gamma appears to be dose-dependent and TNF-alpha acts synergitically with IFN-gamma in the activation of macrophages. The production of reactive NO could be an important effector in the IFN-gamma primed anti-Toxoplasma action. |
| |
| Keywords: | Toxoplasma gondii interferon γ tumor necrosis factor α marcrophage nitric oxide |
| 本文献已被 维普 等数据库收录！ |
| 点击此处可从《中国寄生虫学与寄生虫病杂志》浏览原始摘要信息 |
|
点击此处可从《中国寄生虫学与寄生虫病杂志》下载免费的PDF全文 |
