Coronin-1A Links Cytoskeleton Dynamics to TCRαβ-Induced Cell Signaling |
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| Authors: | B n dicte Mugnier, B atrice Nal, Christophe Verthuy, Claude Boyer, David Lam, Lionel Chasson, Vincent Nieoullon, Genevi ve Chazal, Xiao-Jun Guo, Hai-Tao He, Dominique Rueff-Juy, Andr s Alcover, Pierre Ferrier |
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| Affiliation: | Bénédicte Mugnier, Béatrice Nal, Christophe Verthuy, Claude Boyer, David Lam, Lionel Chasson, Vincent Nieoullon, Geneviève Chazal, Xiao-Jun Guo, Hai-Tao He, Dominique Rueff-Juy, Andrés Alcover, and Pierre Ferrier |
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| Abstract: | Actin polymerization plays a critical role in activated T lymphocytes both in regulating T cell receptor (TCR)-induced immunological synapse (IS) formation and signaling. Using gene targeting, we demonstrate that the hematopoietic specific, actin- and Arp2/3 complex-binding protein coronin-1A contributes to both processes. Coronin-1A-deficient mice specifically showed alterations in terminal development and the survival of αβT cells, together with defects in cell activation and cytokine production following TCR triggering. The mutant T cells further displayed excessive accumulation yet reduced dynamics of F-actin and the WASP-Arp2/3 machinery at the IS, correlating with extended cell-cell contact. Cell signaling was also affected with the basal activation of the stress kinases sAPK/JNK1/2; and deficits in TCR-induced Ca2+ influx and phosphorylation and degradation of the inhibitor of NF-κB (IκB). Coronin-1A therefore links cytoskeleton plasticity with the functioning of discrete TCR signaling components. This function may be required to adjust TCR responses to selecting ligands accounting in part for the homeostasis defect that impacts αβT cells in coronin-1A deficient mice, with the exclusion of other lympho/hematopoietic lineages. |
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