|
|||||
|
|
| 抑癌基因PTEN在鼻咽癌中的表达及突变 | |
| 引用本文： | 程金妹,谢振宇,林勤,张鹏飞,林英,易自翔,田为中. 抑癌基因PTEN在鼻咽癌中的表达及突变[J]. 山东大学耳鼻喉眼学报, 2008, 22(5): 405-407 |
| 作者姓名： | 程金妹 谢振宇 林勤 张鹏飞 林英 易自翔 田为中 |
| 作者单位： | 福建医科大学附属第一医院耳鼻咽喉科,福州,350005;福建医科大学基础医学院病理学系,福州,350004 |
| 基金项目： | 福建省卫生厅青年科研项目，福建省教授基金 |
| 摘 要： | 了解抑癌基因PTEN在鼻咽癌中的表达、突变情况，探讨其与鼻咽癌发生发展的关系。方法采用免疫组化检测52例鼻咽癌中PTEN基因的表达，利用PCR-SSCP银染、DNA测序分析等方法检测PTEN基因第5、8外显子突变。结果鼻咽癌中PTEN蛋白表达率低于癌旁组织，表达与临床TNM分期、颈部淋巴结转移密切相关（P＜0.05）。52例鼻咽癌中PTEN基因第5、8外显子未见明显突变。结论抑癌基因PTEN可能参与鼻咽癌的发生发展，其第5、8外显子在鼻咽癌的发生发展中可能不起关键作用。 |
| 关 键 词： | 鼻咽肿瘤 基因 PIEN 点突变 免疫组织化学 |
Expression and mutation analysis of tumor suppressor PTEN in nasopharyngeal carcinoma |
|
| CHENG Jin-mei,XIE Zhen-yu,LIN Qin,ZHANG Peng-fei,LIN Ying,YI Zi-xiang,TIAN Wei-zhong. Expression and mutation analysis of tumor suppressor PTEN in nasopharyngeal carcinoma[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2008, 22(5): 405-407 | |
| Authors: | CHENG Jin-mei XIE Zhen-yu LIN Qin ZHANG Peng-fei LIN Ying YI Zi-xiang TIAN Wei-zhong |
| Affiliation: | 1. Department of Otolaryngology, First Affiliated Hospital of Fujian Medical University， Fuzhou 350005， China； 2. Department of Pathology, School of Basic Medical Sciences, Fujian Medical University， Fuzhou 350004， China |
| Abstract: | To detect the expression and mutation of tumor suppressor PTEN in nasopharyngeal carcinoma（NPC）， and to investigate the relationship of the PTEN gene to pathogenesis and development of NPC． MethodsExpression levels of PTEN in 52 cases of NPC were determined by an immunohistochemistry method， and polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP), while silver staining and DNA sequencing were used to detect the mutation in exon 5 and 8 of the PTEN gene in NPC. ResultsDifferences of the PTEN expression were significant between NPC and nasopharyngitis (P＜0.05)． Expressions of PTEN were closely related to lymph node metastasis and clinical TNM stage(P＜0.05). None of 52 cases showed mutation in exon5 and 8 of the PTEN gene in NPC． ConclusionPTEN may have some effects on the pathogenesis and development of NPC, and mutation in exon 5 and 8 of PTEN may not play a main role in the genesis and development of NPC. |
| Keywords: | Nasopharyngeal neoplasms Genes PTEN Point mutation Immunohistochemistry |
| 本文献已被 维普 万方数据 等数据库收录！ | |
| 点击此处可从《山东大学耳鼻喉眼学报》浏览原始摘要信息 | |
| 点击此处可从《山东大学耳鼻喉眼学报》下载全文 | |