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| 雷帕霉素联合未成熟树突状细胞诱导小鼠皮肤移植免疫耐受 | |
| 引用本文： | 余思,何晓顺,胡安斌,付必莽,马毅. 雷帕霉素联合未成熟树突状细胞诱导小鼠皮肤移植免疫耐受[J]. 南方医科大学学报, 2008, 28(3): 399-402 |
| 作者姓名： | 余思 何晓顺 胡安斌 付必莽 马毅 |
| 作者单位： | 中山大学附属第一医院器官移植科,广东,广州,510080;中山大学附属第一医院器官移植科,广东,广州,510080;中山大学附属第一医院器官移植科,广东,广州,510080;中山大学附属第一医院器官移植科,广东,广州,510080;中山大学附属第一医院器官移植科,广东,广州,510080 |
| 摘 要： | 目的 研究雷帕霉素联合供者骨髓来源的未成熟树突状细胞在诱导小鼠同种异基因皮肤移植免疫耐受中的协同作用.方法 健康雄性C57BL/6小鼠和BALB/C小鼠分别为供者、受者,建立同种异基因皮肤移植模型.对照组术前术后未给予任何免疫干预措施;雷帕霉素组术后第0天至第6天给予雷帕霉素灌胃;未成熟树突状细胞组将供者骨髓来源的未成熟树突状细胞于术前第7天经尾静脉输注给受者;联合组将供者骨髓来源的未成熟树突状细胞于术前第7d经尾静脉输注给受者,并在术后第0天至第6天给予雷帕霉素灌胃.结果 对照组、雷帕霉素组、未成熟树突状细胞组、联合组的皮肤移植物存活时间分别为(6.9±1.9)、(12.3±3.0)、(17.0±3.4)、(20.8±3.6)d.方差分析提示组间差异有显著性意义(P<0.05);SNK检验提示各组之间的差异均有显著意义.结论 雷帕霉素能延长小鼠皮肤移植物的存活时间;联合供者骨髓来源的未成熟树突状细胞可延长免疫耐受的持续时间. |
| 关 键 词： | 雷帕霉素 未成熟树突状细胞 免疫耐受 小鼠 皮肤移植 |
| 文章编号： | 1673-4254(2008)03-0399-04 |
| 修稿时间： | 2007-11-07 |
Rapamycin combined with donor bone marrow-derived immature dendritic cells induces mouse skin allograft tolerance |
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| YU Si,HE Xiaoshun,HU Anbin,FU Bi-mang,MA Yi. Rapamycin combined with donor bone marrow-derived immature dendritic cells induces mouse skin allograft tolerance[J]. Journal of Southern Medical University, 2008, 28(3): 399-402 | |
| Authors: | YU Si HE Xiaoshun HU Anbin FU Bi-mang MA Yi |
| Affiliation: | Department of Organ Transplantation, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China. |
| Abstract: | OBJECTIVE: To investigate the synergic effects of rapamycin and donor bone marrow-derived immature dendritic cells (DCs) in inducing skin allograft tolerance in mice. METHODS: The recipient BALB/c mice receiving transplantation of skin allograft from C57BL/6 mice were divided into control group (without perioperative treatments), rapamycin group (receiving rapamycin at 1 mg.kg(-1).d(-1) by gavage for 7 consecutive 7 days after skin transplantation), immature DC group (receiving an injection of donor bone marrow-derived immature DCs of 2 x 10(6) via tail vein before skin transplantation), combined group (receiving an injection of the DCs of 2 x 10(6) before transplantation and rapamycin at 1 mg.kg(-1).d(-1) for 7 consecutive days after transplantation). The survival time of the skin allograft was observed in each group. RESULTS: The survival time of the skin allograft in the control, rapamycin, immature DC and immature DC +rapamycin groups were 6.9-/+1.9, 12.3-/+3.0, 17.0-/+3.4 and 20.8-/+3.6 days, respectively, showing significant differences among the groups (P<0.05), and SNK test also indicated significant differences between every two groups. CONCLUSIONS: Rapamycin and donor bone marrow-derived immature DCs have synergic effects in inducing skin allograft tolerance in mice. |
| Keywords: | rapamycin immature dendritic cells immune tolerance mouse skin transplantation |
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