Enzyme-linked immunosorbent assay for detection of Staphylococcus aureus alpha-toxin. |
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| Authors: | O P Surujballi and H B Fackrell |
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| Abstract: | A sandwich enzyme-linked immunosorbent assay was developed for measuring Staphylococcus aureus alpha-toxin. This assay was 500 to 1,000 times more sensitive than the commonly used hemolytic titration assay and was less variable. The binding of alpha-toxin to the adsorbed antibody was most effective after an overnight incubation at 27 degrees C. The toxin was detectable even at a log2 17 dilution of an S. aureus culture supernatant. |
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