| 咬合创伤对大鼠髁突生长发育影响的实验研究 |
| |
| 引用本文： | 陈建中,谢亮焜,王维琦,代自超,杨荣强,张丽,贾玲. 咬合创伤对大鼠髁突生长发育影响的实验研究[J]. 昆明医科大学学报, 2017, 38(10): 1-5 |
| |
| 作者姓名： | 陈建中 谢亮焜 王维琦 代自超 杨荣强 张丽 贾玲 |
| |
| 作者单位： | 1. 昆明医科大学附属口腔医院 |
| |
| 基金项目： | 基金: 国家自然科学基金资助项目 (81360136); 云南省内设机构项目 (2014NS291); 云南省科技厅-昆明医科大学应用基础研究基金资助项目 (2013FB176); |
| |
| 摘 要： | 目的 探讨咬合创伤是否会影响髁突的发育.方法 采用4周龄SD大鼠18只, 随机分为2组, 实验组在左侧上颌磨牙粘结直径为1 mm的钢丝, 对照组不做处理, 于1, 3, 5周处死大鼠, 取实验组双侧及对照组左侧髁突做组织切片.组织学观察髁突形态变化, 免疫组化和TUNEL检测髁突形态及软骨细胞增殖和凋亡情况.结果 组织学显示1, 3, 5周实验组双侧关节与对照组无明显差异.免疫组化和TUNEL显示1, 5周时, 实验组创伤侧与非创伤侧及对照组软骨细胞增殖与凋亡差异无统计学意义 (P>0.05) ;3周时, 创伤侧软骨细胞增殖数减少, 与非创伤侧及对照组相比差异有统计学意义 (P<0.05) .创伤侧软骨细胞凋亡数增加, 与非创伤侧及对照组相比差异有统计学意义 (P<0.05) .结论 一侧咬合创伤对同侧髁突从形态上没有明显影响, 但在细胞生长方面有一定影响, 提示一定程度的咬合创伤不足以对生长发育期的髁突产生明显的影响.
|
| 关 键 词： | 咬合创伤 髁突 发育 |
| 收稿时间： | 2017-05-14 |
The Effect Study of Traumatic Occlusion on Rat Condylar Growth |
| |
| Abstract: | Objective To investigate the effect of traumatic occlusion on the development of condylar.Methods Twenty-seven male SD rats were randomly divided into three groups, the traumatic side, the opposite side and blank control group. The rats of experiment group were perpormed to bond 1 mm steel-wire on left upper molar, the blank group were breeding normally. The condylar was dissected when the rats were 1, 3, 5 weeks after experiment. The histology section was used to observe the morphology of condylar. The immunohistochemical staining was used to examine the PCNA positive cell, TUNEL was chosen to detect the apoptotic cell.Results The histology section showed there was no significant difference between the three groups, the immunochemical stain and TUNEL showed there was no significant difference in the proliferated and apoptotic cells with 1, 5 week after experiment; the proliferated cell number decreased while the apoptotic cell increased on the traumatic side with 3 weeks after experiment, there was significant difference between three groups. Conclusion One side traumatic occlusion has no significant effect on condylar morphology, however has some influence on cell growth, it suggests that traumatic occlusion to some extent has no obvious effect on condylar growth. |
| |
| Keywords: | |
|
| 点击此处可从《昆明医科大学学报》浏览原始摘要信息 |
|
点击此处可从《昆明医科大学学报》下载免费的PDF全文 |
|
