Effect of platelet‐rich plasma (PRP) concentration on proliferation,neurotrophic function and migration of Schwann cells in vitro |
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| Authors: | Canbin Zheng Qingtang Zhu Xiaolin Liu Xijun Huang Caifeng He Li Jiang Daping Quan Xiang Zhou Zhaowei Zhu |
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| Affiliation: | 1. Department of Orthopaedics and Microsurgery, First Affiliated Hospital of Sun Yat‐Sen University, Guangzhou, People's Republic of China;2. Guangzhou ZhongDa Medical Equipment Co. Ltd, Guangzhou, People's Republic of China;3. Orthopaedic Institute of the First Affiliated Hospital, Sun Yat‐Sen University, Guangzhou, People's Republic of China;4. School of Chemistry and Chemical Engineering, Sun Yat‐Sen University, Guangzhou, People's Republic of China |
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| Abstract: | Platelet‐rich plasma (PRP) contains various growth factors and appears to have the potential to promote peripheral nerve regeneration, but evidence is lacking regarding its biological effect on Schwann cells (SCs). The present study was designed to investigate the effect of PRP concentration on SCs in order to determine the plausibility of using this plasma‐derived therapy for peripheral nerve injury. PRP was obtained from rats by double‐step centrifugation and was characterized by determining platelet numbers and growth factor concentrations. Primary cultures of rat SCs were exposed to various concentrations of PRP (40%, 20%, 10%, 5% and 2.5%). Cell proliferation assays and flow cytometry were performed to study to assess SC proliferation. Quantitative real‐time PCR and ELISA analysis were performed to determine the ability of PRP to induce SCs to produce nerve growth factor (NGF) and glial cell line‐derived neurotrophic factor (GDNF). Microchemotaxis assay was used to analyse the cell migration capacity. The results obtained indicated that the platelet concentration and growth factors in our PRP preparations were significantly higher than in whole blood. Cell culture experiments showed that 2.5–20% PRP significantly stimulated SC proliferation and migration compared to untreated controls in a dose‐dependent manner. In addition, the expression and secretion of NGF and GDNF were significantly increased. However, the above effects of SCs were suppressed by high PRP concentrations (40%). In conclusion, the appropriate concentration of PRP had the potency to stimulate cell proliferation, induced the synthesis of neurotrophic factors and significantly increased migration of SCs dose‐dependently. Copyright © 2013 John Wiley & Sons, Ltd. |
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| Keywords: | platelet‐rich plasma growth factors Schwann cells cell proliferation neurotrophic function migration |
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