Isolation of influenza virus in human lung embryonated fibroblast cells (MRC-5) from clinical samples. |
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| Authors: | M de O a, S Mel n, P de la Iglesia, F Hidalgo, A F Verdugo |
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| Affiliation: | M de Oña, S Melón, P de la Iglesia, F Hidalgo, and A F Verdugo |
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| Abstract: | Ninety-four pharyngeal swab samples corresponding to 94 patients with suspected influenza virus infection were inoculated in Madin-Darby canine kidney (MDCK) cells, the conventional cell system for the isolation of influenza virus, and in fibroblastic human embryo lung (MRC-5) cells, a cell system less commonly used for this purpose but one frequently used in clinical virology laboratories. Both cell preparations were treated with trypsin. Influenza virus was recovered from 15% of the samples inoculated in MDCK cells and from 18% of those inoculated in MRC-5 cells. The use of MRC-5 cells can simplify the search for respiratory viruses and would assist in the rapid detection of influenza virus during new epidemics. |
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