Oral l‐glutamine administration attenuated cutaneous wound healing in Wistar rats |
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| Authors: | Saurabh Goswami Amit Kandhare Anand A Zanwar Mahabaleshwar V Hegde Subhash L Bodhankar Sudhir Shinde Shahaji Deshmukh Ravindran Kharat |
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| Affiliation: | 1. Department of Surgery, Bharati Vidyapeeth Deemed University Medical College, Pune, India;2. Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Pune, India;3. Center for Innovation in Nutrition Health Disease, Interactive Research School for Health Affairs, Medical College Campus, Bharati Vidyapeeth Deemed University, Pune, India |
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| Abstract: | The objective of this study was to evaluate the wound healing potential of l ‐glutamine in laboratory rats using excision and incision wound models. Excision wounds of size 500 mm2 and depth 2 mm were made on the dorsal portion of male Wistar rats (230–250 g) and were used for the study of oral l ‐glutamine (1 g/kg) treatment on the rate of contraction of wound and epithelisation. Histological evaluation of wound tissue was also performed. Six‐centimetre‐long two linear‐paravertebral incisions in male Wistar rats (230–250 g) were used to study the effect of l ‐glutamine (1 g/kg, p.o.) treatment on tensile strength, total protein and hydroxyproline content in the incision model. Oral administration of l ‐glutamine (1 g/kg) significantly decreased wound area, epithelisation period and wound index, whereas the rate of wound contraction significantly increased (P < 0·001) when compared with vehicle control rats in the excision wound model. Tensile strength, hydroxyproline content and protein level were significantly increased (P < 0·001) in l ‐glutamine (1 g/kg, p.o.)‐treated rats when compared with vehicle control rats in the incision wound model. Histological evaluation of wound tissue from l ‐glutamine (1 g/kg, p.o.)‐treated rats showed complete epithelialisation with new blood vessel formation and high fibrous tissues in the excision wound model. In conclusion, oral administration of l ‐glutamine (1 g/kg) promotes wound healing by acting on various stages of wound healing such as collagen synthesis, wound contraction and epithelialisation. |
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| Keywords: | Excision wounds Hydroxyproline Incision wounds l‐Glutamine Tensile strength |
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