MiR-126 Modulates Angiogenesis in Breast Cancer by Targeting VEGF-A -mRNA |
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| Authors: | Layla Alhasan |
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| Affiliation: | Department of Biology, College Education for Pure Sciences, Thi-Qar University, Nasiriya, Iraq. |
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| Abstract: | Background: Breast cancer is most serious reasons of women death around worldwide result in increasing itsmorbidity and mortality. MicroRNAs are considered as significant regulators of cancer biological processes. The mainaim of this study is restoration of miR-126 could lead to modulate breast cell line and impairs their proliferation bytargeting vascular endothelial growth factor gene (VEGF-A). Methods: Breast cancer cell line (MCF7) was transfectedby miR-126 lipofectamine and negative miR control for 24 hr. Cytotoxic effects of miR-126 lipofectamine weredetermined by cell viability assay. Cell proliferation and cell cycle were quantitatively measured using PicoGreenassay and DAPI stain-flow cytometer analysis. For further investigation, Taq-Man real time PCR assay was performedto detect relative VEGF-A and miRNA-126 level. Results: MiR-126 was overexpressed in treated breast cancer cell(MCF7) compared with control cells. miR-126 expression has been associated –with a decrease in cell proliferationand arrested MCF7 cells at G1 phase. The study found that vascular endothelial growth factor is regulated by miR-126. Hence, VEGF-A is considered as functional vital and direct target to miR-126 in breast cancer cell line (MCF7).Conclusions: This study provided that manipulated miR-126 level may suggest a novel therapeutic approach in breastcancer treatment. However, an animal models study is needed to address and prove predictive ability of miR-126 onbreast cancer controlling. |
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| Keywords: | miR-126 Lipofectamine MCF7 cells VEGF-A expression Cell cycle Taqman real time PCR assay |
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