Detection of Epidermal Growth Factor Receptor (EGFR) Gene Mutation in Formalin Fixed Paraffin Embedded Tissue by Polymerase Chain Reaction-Single Strand Conformational Polymorphism (PCR-SSCP) in Non-Small Cell Lung Cancer in the Northeastern Region of Thailand |
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| Authors: | Kornsiri Saiyaros1Panutus Kritpetcharat1Chawalit Pairojkul2Jiraporn Sitthithaworn1 3 |
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| Affiliation: | 1Department of Clinical Microscopy, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen,Thailand.2Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.3Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand. |
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| Abstract: | Background: The use of targeted specific genes in therapeutic and treatment decisions has been considered forlung cancer. The epidermal growth factor receptor (EGFR) gene, which is over expressed in non-small cell lung cancer(NSCLC), was considered as one of the targeted specific genes. EGFR mutations in exons 18–21, which encode aportion of the EGFR kinase domain, were found in NSCLC patients and were associated with the response of EGFRtyrosinekinase inhibitors (EGFR-TKIs). Therefore, a molecular technique for EGFR mutation detection has importantbenefits for therapy in NSCLC patients. This study aims to determine the EGFR mutations in patients with NSCLCusing polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) in exons 18-21. Methods:DNA samples were extracted from formalin fixed paraffin embedded tissues of NSCLC patients who attended hospital.The extracted DNA was used as a template for the EGFR gene amplification. Results: Occurrence of EGFR mutationswere found in 29 out of 50 cases (58%).The frequency of EGFR mutations by first PCR at exon 18, 19, 20 and 21were 6 (12%), 19 (38%) 20 (40%) and at 21 (42%), respectively. By PCR-SSCP, the frequencies of EGFR mutationsat exon 18, 19, 20 and 21 were 3(6%), 18(36%), 23(46%) and 13(26%), respectively. All of the mutations found werein agreement with DNA sequencings. Conclusion: The high frequency of EGFR mutations in NSCLC suggests thatPCR-SSCP is a efficient screening method and useful for treatment plan. |
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| Keywords: | Epidermal growth factor receptor (EGFR) Mutation Non-small cell lung cancer (NSCLC) polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) |
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