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| IL-10在感染日本血吸虫p47 GTP酶缺失小鼠中的免疫调节作用 | |
| 引用本文： | 吴婧娇,田芳,高雅楠,杜晓棠,张美娟,季旻珺,吴观陵. IL-10在感染日本血吸虫p47 GTP酶缺失小鼠中的免疫调节作用[J]. 中国血吸虫病防治杂志, 2010, 22(2): 111-116 |
| 作者姓名： | 吴婧娇 田芳 高雅楠 杜晓棠 张美娟 季旻珺 吴观陵 |
| 作者单位： | 南京医科大学病原生物学系（南京 210029） |
| 摘 要： | 目的在日本血吸虫早期感染模型和SEA致敏模型中,研究IGTP和IRG-47缺失小鼠在抵抗日本血吸虫感染过程中IL-10的免疫调节作用。方法以IGTP基因敲除(IGTP-/-)小鼠、IRG-47基因敲除(IRG-47-/-)小鼠和野生型(WT)小鼠为研究对象,建立日本血吸虫早期感染模型和可溶性虫卵抗原(SEA)致敏模型。在早期感染模型中,以HE染色观察耳廓的病理变化,间接ELISA法检测耳廓皮片培养上清中Th1/Th2型细胞因子的表达水平;在SEA致敏模型中,以间接ELISA法检测血清中日本血吸虫特异性IgG抗体、脾单个核细胞培养上清中Th1/Th2型细胞因子的表达水平;以及流式细胞术检测脾细胞亚群比例的变化。结果日本血吸虫感染7d后,IGTP-/-、IRG-47-/-和WT小鼠的耳廓病理变化无显著差异;但IRG-47-/-小鼠耳廓培养上清中IL-10水平显著低于IGTP-/-小鼠。SEA致敏小鼠3周后,IGTP-/-小鼠血清中SEA特异性IgG抗体水平显著低于WT小鼠;IRG-47-/-小鼠脾细胞在体外SEA刺激下,产生的IL-10水平显著高于IGTP-/-小鼠和WT小鼠;流式结果显示IRG-47-/-小鼠脾中Th2细胞比例显著低于WT小鼠。结论SEA是IRG-47缺失小鼠抵抗日本血吸虫、促使宿主产生保护性应答的重要调节因素,IL-10在此过程中是重要的免疫调节分子。 |
| 关 键 词： | 日本血吸虫 p47 GTP酶 免疫调节 IL-10 基因敲除 小鼠 |
Immune regulation of IL-10 to Schistosoma japonicum infection in p47 GTPase deficient mouse model |
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| Wu Jing-jiao,Tian Fang,Gao Ya-nan,Du Xiao-tang,Zhang Mei-juan,Ji Min-jun,Wu Guan-ling. Immune regulation of IL-10 to Schistosoma japonicum infection in p47 GTPase deficient mouse model[J]. Chinese journal of schistosomiasis control, 2010, 22(2): 111-116 | |
| Authors: | Wu Jing-jiao Tian Fang Gao Ya-nan Du Xiao-tang Zhang Mei-juan Ji Min-jun Wu Guan-ling |
| Affiliation: | Department of Pathogenic Biology, Nanjing Medical University, Nanjing 214064, China |
| Abstract: | Objective To investigate the immune response of IL-10 to Schistosoma japonicum infection in the early infectioin model and SEA immunization model of the IGTP~(-/-) and IRG-47~(-/-) mice.Methods In the early infection model,the IGTP knock out (IGTP~(-/-)) mice,IRG-47 knock out (IRG-47~(-/-)) mice and wild-type (WT) C57BL/6J mice were exposed to 300 S.japonicum cercariae via the pinna and sacrificed on day 7 post-infection.Each mouse pinna section was stained with hematoxylin-eosin (HE) to detect the pathological lesions,and the culture supernatant of pinna was used to test the levels of Th1/Th2 cytokines by indirect ELISA.In the SEA immunization model,IGTP~(-/-) IRG-47~(-/-) and WT mice were immunized with SEA twice and sacrificed in 3 weeks after the initial immunization.SEA-specific IgG antibody in sera was detected by indirect ELISA;the levels of Th1/Th2 cytokines were tested in culture supernatant of splenocytes by indirect ELISA;the proportions of CD4~+ T cells,CD8~+ T cells,B cells,Th1 and Th2 cells in the spleen were assayed by FACS.Results Although no obvious differences on the pathology of pinna were observed among the three mouse groups,the level of IL-10 in the culture supernatant of pinna of IRG-47~(-/-) mice was lower than that of IGTP~(-/-) mice in 7 days after the exposure.Following SEA immunization,the level of SEA-specific IgG antibody in sera of IGTP~(-/-) mice was lower than that in WT mice,the level of IL-10 in the culture supernatant of splenocytes of IRG-47~(-/-) mice was higher than that of IGTP~(-/-) and WT mice with the stimulation of SEA.However,the proportion of Th2 cells in the spleen of IRG47~(-/-) mice was the lowest among the three mouse groups.Conclusions SEA is the stimulus of IRG-47 deficiency mice to defend Schistosoma japanicum infection and promote the host to produce a protective response,and IL-10 may play an important role in immune regulation in this process. |
| Keywords: | Schistosoma japonicum p47 GTPase Regulation of immune response IL-10 Knock out Mouse |
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