| 白细胞介素-10对人牙龈成纤维细胞分泌白细胞介素-1β的抑制作用 |
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| 引用本文： | 于新波,杨丕山,葛少华. 白细胞介素-10对人牙龈成纤维细胞分泌白细胞介素-1β的抑制作用[J]. 华西口腔医学杂志, 2002, 20(4): 238-240 |
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| 作者姓名： | 于新波 杨丕山 葛少华 |
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| 作者单位： | 250012 山东大学口腔医学院(于新波现在青岛大学医学院) |
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| 摘 要： | 目的:探讨白细胞介素-10(IL-10)在牙周炎症反应中的抑制作用。方法:用细胞培养法、酶联免疫吸附分析法 (ELISA)检测细胞培养液上清中的IL-1B浓度;用MTT法检测人牙龈成纤维细胞(HGF)对细菌脂多糖(LPS)、IL-10的增殖反应。结果:LPS以剂量依赖方式诱导HGF产生IL-1B,6 h达最大分泌量;IL-1B的分泌可被IL-10抑制,10 ng/ ml IL-10作用24 h抑制效应达最大;LPS对HGF具有促增殖作用,而IL-10对HGF的增殖无影响。结论:IL-10是一种抑制牙周炎症反应的细胞因子。
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| 关 键 词： | 白细胞介素类 人牙龈成纤维细胞 牙周炎 |
| 收稿时间： | 2002-08-25 |
| 修稿时间： | 2000-09-14 |
Inhibitory Effects of Interleukin-10 on the Production of Interleukin-1β by Human Gingival Fibroblasts |
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| Yu Xinbo,Yang Pishan,Ge Shaohua,College of Stomatology,Shandong University,. Inhibitory Effects of Interleukin-10 on the Production of Interleukin-1β by Human Gingival Fibroblasts[J]. West China journal of stomatology, 2002, 20(4): 238-240 |
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| Authors: | Yu Xinbo Yang Pishan Ge Shaohua College of Stomatology Shandong University |
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| Affiliation: | Yu Xinbo, Yang Pishan, Ge Shaohua College ofStomatology, ShandongUniversity |
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| Abstract: | Objective:The purpose of this study was to investigate the inhibitory effect of interleukin-10 (IL-10) on the periodontal inflammatory reaction.Methods:Cell culture and ELISAwere used to evaluate the concentration of interleukin-1B(IL-1B) in the supernatant, and MTT was used to examine the proliferation response of human gingival fibroblasts to lipopolysaccharides and IL-10.Results:IL-1Bsecreted by human gingival fibroblastswas induced by lipopolysaccharides in a dose-dependentmanner and reached the highest level in 6 h. IL-1Bwas inhibited by IL-10, and the optimal inhibitory concentration of latter was 10 ng/ml. The inhibitory effect of IL-10 in the optimal concentration to IL-1Breached the maximum in 24 h. Further the proliferation of hu- man gingival fibroblasts was also induced by lipopolysaccharides, but not affected by IL-10.Conclusion:IL-10 is a cytokine that can inhibit periodontal inflammatory reaction. |
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| Keywords: | interleukin human gingival fibroblasts periodontitis |
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