| 血清重轻链检测在浆细胞病中的应用 |
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| 引用本文： | 张聪丽, 王琛, 李剑. 血清重轻链检测在浆细胞病中的应用[J]. 中国肿瘤临床, 2016, 43(15): 646-649. DOI: 10.3969/j.issn.1000-8179.2016.15.479 |
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| 作者姓名： | 张聪丽 王琛 李剑 |
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| 作者单位： | 作者单位:中国医学科学院,北京协和医院血液内科(北京市100730) |
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| 摘 要： | 单克隆免疫球蛋白（M 蛋白）是单克隆浆细胞增殖性疾病诊断、预后分析及疗效评价的核心指标。目前，临床上主要应用血清蛋白电泳及免疫固定电泳对其进行测定。然而血清蛋白电泳对于含量较低或移动异常的M 蛋白难以定量，而免疫固定电泳只能对M 蛋白进行定性分型。血清重轻链（heavyandlightchain，HLC ）检测，作为一种新兴的M 蛋白定量技术，可以特异性测定重链及轻链相关的免疫球蛋白水平。进一步计算HLC 的比值，则可以反映疾病的单克隆浆细胞增殖程度。近年来，HLC 检测已被应用于多种单克隆浆细胞增殖性疾病的研究，给此类疾病的诊疗带来新的检测手段。本文就HLC 检测在该类疾病中的应用进行综述。
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| 关 键 词： | 单克隆免疫球蛋白 血清重轻链 单克隆浆细胞增殖性疾病 |
| 收稿时间： | 2016-04-26 |
| 修稿时间： | 2016-06-07 |
Measurement of serum heavy/light chain in monoclonal plasma cell proliferative disor-ders |
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| Congli ZHANG, Chen WANG, Jian LI. Measurement of serum heavy/light chain in monoclonal plasma cell proliferative disorders[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2016, 43(15): 646-649. DOI: 10.3969/j.issn.1000-8179.2016.15.479 |
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| Authors: | Congli ZHANG Chen WANG Jian LI |
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| Affiliation: | Department of Hematology, Peking Union Medical College Hospital, Beijing100730, China |
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| Abstract: | Monoclonal protein (M protein) is a serum surrogate used to conduct diagnostic, prognostic, and therapeutic evaluations of monoclonal plasma cell proliferative disorders. Two basic methods, namely, serum protein electrophoresis and immunofixation elec-trophoresis, are employed to detect and characterize M protein. Although these techniques have considerably improved, M protein quantification exhibits several drawbacks. In serum protein electrophoresis, M protein can migrate to various locations, and low M pro-tein levels cannot form a typical peak;as a consequence, additional problems in measurements arise. In 2009, a novel immunoassay in-volving a heavy/light chain (HLC) was developed. HLC recognizes immunoglobulins with specific heavy and light chain isotypes. The ra-tio between an involved monoclonal immunoglobulin and an uninvolved background polyclonal immunoglobulin can be calculated through immunoglobulin quantitation by using isotype-specific light chains. This review summarizes relevant parameters that provide diagnostic, prognostic, and therapeutic data regarding monoclonal plasma cell proliferative disorders. |
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| Keywords: | monoclonal protein serum heavy/light chain monoclonal plasma cell proliferative disorders |
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