| C57-ras癌症转基因小鼠模型胚胎冷冻保种技术研究 |
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| 引用本文： | 左琴,刘甦苏,周舒雅,刘佐民,王金恒,范昌发. C57-ras癌症转基因小鼠模型胚胎冷冻保种技术研究[J]. 中国比较医学杂志, 2013, 23(5): 34-37 |
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| 作者姓名： | 左琴 刘甦苏 周舒雅 刘佐民 王金恒 范昌发 |
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| 作者单位： | 左琴 (中国食品药品检定研究院,国家啮齿类实验动物种子中心,北京 100050)； 刘甦苏 (中国食品药品检定研究院,国家啮齿类实验动物种子中心,北京 100050)； 周舒雅 (中国食品药品检定研究院,国家啮齿类实验动物种子中心,北京 100050)； 刘佐民 (中国食品药品检定研究院,国家啮齿类实验动物种子中心,北京 100050)； 王金恒 (中国食品药品检定研究院,国家啮齿类实验动物种子中心,北京 100050)； 范昌发 (中国食品药品检定研究院,国家啮齿类实验动物种子中心,北京 100050)； |
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| 摘 要： | 目的 建立C57-ras癌症转基因小鼠模型胚胎的冷冻保种技术.方法 通过直接收集体内发育的2-细胞胚或者通过体外受精方法获得2-细胞胚,采用玻璃化冷冻法冷冻、复苏、移植,后代经PCR检测.结果 体内发育至2细胞期胚胎90枚,解冻后的平均复苏率是67.78％,移植产仔率是21.31％,后代的阳性率是23.08％；体外受精的2细胞期胚胎203枚,解冻复苏60枚,平均复苏率是63.33％,移植产仔率是15.78％,后代的阳性率是33.33％.结论 两种胚胎冷冻途径均获得了C57-ras癌症转基因动物模型阳性小鼠,建立了该模型动物的低温保存方法.
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| 关 键 词： | C57-ras转基因小鼠 体外受精 胚胎冷冻 2细胞期胚胎 |
Study on the in vitro Fertilization and Embryo Cryopreservation in Carcinogenesis Transgenic C57-ras Mouse Model |
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| ZUO Qin,LIU Su-su,ZHOU Shu-ya,LIU Zuo-min,Wang Jin-hen,FAN Chang-fa. Study on the in vitro Fertilization and Embryo Cryopreservation in Carcinogenesis Transgenic C57-ras Mouse Model[J]. Chinese Journal of Comparative Medicine, 2013, 23(5): 34-37 |
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| Authors: | ZUO Qin LIU Su-su ZHOU Shu-ya LIU Zuo-min Wang Jin-hen FAN Chang-fa |
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| Affiliation: | (National Rodent Laboratory Animal Resource Center,National Institutes Food and Drug Control,Beijing 100050,China) |
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| Abstract: | Objective Investigate the application of in vitro fertilization （IVF） and embryo cryopreservation in C57-ras transgenie mice. Methods 2-cell embryos were collected in two ways, first of which is by 4-week superevulated C57BL/6J female mice mated with C57-ras transgentlc male mice, and the other is by IVF. The 2-cell embryos were cryopreserved after that. Then the cryopreserved 2-cell embryos were thawed and transplanted to caculate the ratio. Genetype of offsprings were analysed by PCR. Results The average recovery rate of cryopreserved normal 2-cell embryo was 67.78% and 21.31% were born after thawed 2-cell embryos transplanted. 23.08% of the offsprings were positive of human e-Ha-ras gene; the average recovery rate of eryopreserved IVF 2-cell was d3.33% , and 15.78% were born after transplanted, and 33.33% of the offspring were positive. Conclusion The results suggested that there was no significant difference in cryopreservation of normal and IVF C57-ras 2-cell embryos. The research provided the eryopreservation method for C57-ras transgenie mouse model. |
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| Keywords: | C57-ras transgenic mouse model In vitro fertilization （IVF） Embryo cryopreservation 2-cell embryo |
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