| 实体瘤患者与健康供者外周血造血干细胞未动员采集因素分析 |
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| 引用本文： | 纪黎明,陆紫敏,方琦,刘承重,李雪晨. 实体瘤患者与健康供者外周血造血干细胞未动员采集因素分析[J]. 中华临床医师杂志(电子版), 2019, 13(6): 419-423. DOI: 10.3877/cma.j.issn.1674-0785.2019.06.003 |
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| 作者姓名： | 纪黎明 陆紫敏 方琦 刘承重 李雪晨 |
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| 作者单位： | 1. 200065 上海，同济大学附属同济医院输血科 |
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| 基金项目： | 上海市卫生局科研课题资助项目(2010046); 上海市公共卫生重点学科建设计划资助(12GWZX0202) |
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| 摘 要： | 目的研究影响外周血造血干细胞（PBSC）未动员的采集因素。 方法对同济大学附属同济医院自2016年1月至2018年6月56例实体瘤组患者及53名健康组供者未采用粒细胞集落刺激因子动员（简称未动员）就使用MCS＋血细胞分离机采集PBSC，比较分析2组采集前白细胞（WBC）计数、红细胞比容（Hct）、血小板（PLT）计数、血红蛋白（Hb）浓度、单个核细胞（MNC）计数及采集效果差异，并分别与部分影响因素进行相关性分析。 结果所有未动员采集者均成功采集到一定数量的MNC，CD34＋细胞。（1）健康组采集前Hct、PLT、Hb指标均高于疾病组［（44.81±3.99）% vs （38.38±4.49）%；（230.91±44.63）×109/kg vs（202.88±60.39）×109/kg；（146.09±16.62）g/L vs （129.07±15.54）g/L］，差异具有统计学意义（t=7.84、2.74、5.51，P<0.05）。（2）健康组采集物中MNC总数、WBC计数均高于疾病组［（2.81±1.35）×108/kg vs（1.99±1.05）×108/kg；（92.23±29.46）×109/L vs（54.60±23.61）×109/L］，CD34＋、MNC却低于疾病组［（1.17±0.66）% vs（1.65±1.01）%；（53.68±20.19）% vs（65.11±16.45）%］，差异均有统计学意义（t=3.54、7.35、2.91、3.24，P<0.05）。（3）健康组采集物CD34＋细胞及MNC总数均与采集前Hct指标成负相关（r=-0.30915、-0.19624，P=0.00004、0.01119），CD34＋细胞数与采集血总容量、采集者体质量成负相关（r=-0.04114、-0.15436，P=0.02612、0.03071）；（4）疾病组采集物CD34＋细胞及MNC总数与采集前PLT计数、采集者体质量指数成正相关（r=0.14135、0.19609；r=0.04678、0.14765，P<0.05），CD34＋细胞数与采集前WBC计数、体质量成正相关（r=0.02028、0.18605，P<0.05）。 结论所有未动员采集者其PBSC采集效果与采集前WBC和PLT计数、Hct、体质量指数、采集循环血量和成品量均有关联，其中健康组采集物中MNC总数、WBC计数高于疾病组。
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| 关 键 词： | 实体瘤 健康供者 未动员 外周血造血干细胞 采集 |
| 收稿时间： | 2018-09-03 |
Factors influencing collection of non-mobilized peripheral blood stem cells from solid tumor patients and healthy donors |
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| Liming Ji,Zimin Lu,Qi Fang,Chengzhong Liu,Xuechen Li. Factors influencing collection of non-mobilized peripheral blood stem cells from solid tumor patients and healthy donors[J]. Chinese Journal of Clinicians(Electronic Version), 2019, 13(6): 419-423. DOI: 10.3877/cma.j.issn.1674-0785.2019.06.003 |
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| Authors: | Liming Ji Zimin Lu Qi Fang Chengzhong Liu Xuechen Li |
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| Affiliation: | 1. Department of Blood Transfusion, Affiliated Tongji Hospital, Shanghai Tongji University, Shanghai 200065, China |
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| Abstract: | ObjectiveTo investigate the factors that affect the collection of non-mobilized peripheral blood stem cells (PBSCs). MethodsAutologous PBSCs without mobilization with granulocyte colony stimulating factor (G-CSF) were collected from 56 patients with solid tumor and 53 healthy donors at the Affiliated Tongji Hospital of Tongji University from January 2016 to June 2018 using an MCS+ blood cell separator. White blood cells (WBC), hematocrit (Hct), platelets (PLT), hemoglobin (Hb), mononuclear cells (MNC), and collection index before and after collection were compared between the two groups. The correlation between collection effects and some influencing factors was analyzed. ResultsA certain number of MNC and CD34+ cells were successfully collected in both groups. Hct, PLT, and Hb in the healthy group were significantly higher than those in the patient group [(44.81±3.99)% (38.38±4.49)%; (230.91±44.63)×109/kg vs (202.88±60.39)×109/kg; (146.09±16.62)g/L vs (129.07±15.54) g/L; t=7.84, 2.74, 5.51, P<0.05)]. The total number of MNC and WBC in the control group was significantly higher than that in the patient group [(2.81±1.35)×108/kg vs (1.99±1.05)×108/kg; (92.23±29.46)×109/L vs (54.60±23.61)×109/L; t=3.54, 7.35, P<0.05], but the percentages of CD34+ cells and MNC were significantly lower than those in the patient group [(1.17±0.66)% vs (1.65±1.01)%; (53.68±20.19)% vs (65.11±16.45)%; t=2.91, 3.24, P<0.05]. The total number of CD34+ cells and MNC in the healthy group was negatively correlated with the pre-harvest Hct index (r=-0.30915, -0.19624; P=0.00004, 0.01119), and the number of CD34+ cells was negatively correlated with the total volume of blood collected and the body mass of the collected patients (r=-0.04114, -0.15436, P=0.02612, 0.03071). The total number of CD34+ cells and MNC in the patient group was positively correlated with the pre-harvest PLT count and the body mass index (r=0.14135, 0.19609; r=0.04678, 0.14765, P<0.05). The number of CD34+ cells was positively correlated with WBC count and body mass before harvest (r=0.02028, 0.18605, P<0.05). ConclusionThere is a correlation between the collection of non-mobilized PBSCs and WBC, PLT, Hct, body mass index, product volume, and the amount of blood circulation. The total number of MNC and WBC in healthy controls are significantly higher than those in the patient group. |
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| Keywords: | Solid tumor Healthy donor Non-mobilization Peripheral blood stem cells Collection |
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