| 缝隙连接蛋白26在肺腺癌相关成纤维细胞中的表达及临床意义 |
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| 引用本文： | 宋锦静,韦彤彤,梁凯,黄志广,陈罡,茅乃权,阳洁. 缝隙连接蛋白26在肺腺癌相关成纤维细胞中的表达及临床意义[J]. 中国癌症防治杂志, 2021, 13(2): 201-206. DOI: 10.3969/j.issn.1674-5671.2021.02.16 |
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| 作者姓名： | 宋锦静 韦彤彤 梁凯 黄志广 陈罡 茅乃权 阳洁 |
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| 作者单位： | 广西医科大学药学院；广西医科大学附属肿瘤医院胸瘤外科；广西医科大学第一附属医院病理科 |
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| 基金项目： | 国家自然科学基金项目(81860649);广西自然科学基金项目(2018GXNSFAA050053);广西研究生教育创新计划项目(YCBZ2020054)。 |
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| 摘 要： | 目的 探讨缝隙连接蛋白26(connexin 26,Cx26)在肺腺癌相关成纤维细胞中的表达及其临床意义.方法 收集2018年11月至2019年5月于广西医科大学附属肿瘤医院6例手术切除肺腺癌癌组织及相应癌旁组织,分离原代肿瘤相关成纤维细胞(cancer-associated fibroblasts,CAFs)和正常肺...
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| 关 键 词： | 肺腺癌 成纤维细胞 缝隙连接蛋白 肿瘤相关成纤维细胞 正常肺成纤维细胞 |
Expression and clinical significance of connexin 26 in lung adenocarcinoma associated fibroblasts |
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| SONG Jinjing,WEI Tongtong,LIANG Kai,HUANG Zhiguang,CHEN Gang,MAO Naiquan,YANG Jie. Expression and clinical significance of connexin 26 in lung adenocarcinoma associated fibroblasts[J]. Journal of Chinese Medical Abstracts·Oncology, 2021, 13(2): 201-206. DOI: 10.3969/j.issn.1674-5671.2021.02.16 |
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| Authors: | SONG Jinjing WEI Tongtong LIANG Kai HUANG Zhiguang CHEN Gang MAO Naiquan YANG Jie |
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| Affiliation: | (Department of Pharmacology,Guangxi Medical University;Department of Thoracic Tumor Surgery,Guangxi Medical University Cancer Hospital;Department of Pathology,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China) |
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| Abstract: | ObjectiveTo investigate the expression of connexin 26(Cx26)in lung adenocarcinoma associated fibroblasts and its clinical significance.MethodsThe 6 cases of lung adenocarcinoma tissues and adjacent normal lung tissues that were surgically resected in Guangxi Medical University Cancer Hospital from November 2018 to May 2019 were collected,and the primary cancerassociated fibroblasts(CAFs)and normal lung fibroblasts(NLFs)were isolated.The primary fibroblasts were identified through the cell morphology observed by optical microscope,the expression level of cell biomarkersα-SMA,E-cadherin and Vimentin detected by the Western blot and immunofluorescence staining;the level of Cx26 mRNA was detected by qRT-PCR.The distribution and expression of Cx26 in 22 cases of lung adenocarcinoma and adjacent tissues collected from January 2013 to December 2018 were detected by immunofluorescence staining.ResultsThe primary CAFs and NLFs were successfully isolated.The microscopy reported that CAFs and NLFs were spindle-shaped cells,both of which expressedα-SMA and Vimentin,but only slightly expressed E-cadherin,and the expression level ofα-SMA in CAFs was higher than that in NLFs(P<0.001).The qRT-PCR results showed that the expression level of Cx26 in CAFs showed a continuous downward trend with the increase of tumor stage(t=5.684,P=0.011).The immunofluorescence staining results showed that the expression of Cx26 in lung adenocarcinoma tissues was lower than that in normal lung tissues(t=2.609,P=0.016).ConclusionsDownregulation of Cx26 expression in CAFs within tumor microenvironment may be associated with higher stage and progressive malignancy of lung adenocarcinoma. |
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| Keywords: | Lung adenocarcinoma Fibroblasts Connexin CAFs NLFs |
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