| 普罗布考对糖基化终末产物引发心脏微血管内皮功能紊乱的作用 |
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| 引用本文： | 成永霞,刘贵波,于建渤,郭素芬,颜彬,李志强,杨向红. 普罗布考对糖基化终末产物引发心脏微血管内皮功能紊乱的作用[J]. 心脏杂志, 2012, 24(3): 324-327 |
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| 作者姓名： | 成永霞 刘贵波 于建渤 郭素芬 颜彬 李志强 杨向红 |
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| 作者单位： | (牡丹江医学院:1.病理学教研室，2.解剖学教研室，黑龙江 牡丹江157011；3.中国医科大学附属盛京医院病理科，沈阳 100011) |
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| 基金项目： | 黑龙江省青年科学基金项目资助(QC2010040);黑龙江省2011年研究生创新科研资金项目资助(YJSCX2011-299HLJ);牡丹江医学院科学技术研究项目资助(2010-16) |
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| 摘 要： | 目的:观察普罗布考（probucol）对糖基化终末产物（AGEs）作用后的心脏微血管内皮细胞iNOS表达的影响。方法: 原代培养心脏微血管内皮细胞，AGEs（100 mg/L）体外模拟高糖环境，在probucol(5 μmol/L,10 μmol/L,20 μmol/L) 作用后，检测ROS、NO和iNOS变化情况。结果: 与AGEs组比较，probucol组中ROS蛋白表达降低，NO生成增加，而 iNOS蛋白表达降低，且显示有浓度依赖性。结论: probucol可能通过对抗氧化应激的途径，缓解AGEs引发的心脏微血管内皮功能障碍。
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| 关 键 词： | 心脏微血管内皮细胞 糖基化终末产物 普罗布考 诱导型一氧化氮合酶 |
| 收稿时间： | 2011-11-25 |
Effect of probucol on advanced glycosylation end product-induced dysfunction in cardiac microvascular endothelial cells |
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| CHENG Yong-xia,LIU Gui-bo,YU Jian-bo,GUO Su-fen,YAN Bin,Li Zhi-qiang,YANG Xiang-hong. Effect of probucol on advanced glycosylation end product-induced dysfunction in cardiac microvascular endothelial cells[J]. Chinese Heart Journal, 2012, 24(3): 324-327 |
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| Authors: | CHENG Yong-xia LIU Gui-bo YU Jian-bo GUO Su-fen YAN Bin Li Zhi-qiang YANG Xiang-hong |
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| Affiliation: | 1.Department of Pathology,2.Department of Anatomy,Mudanjiang Medical College,Mudanjiang 157011,Heilongjiang,China;3.Department of Pathology,Affiliated Shengjing Hospital,China Medical University,Shenyang 100011,China) |
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| Abstract: | AIM: To observe the effect of probucol on the iNOS expression induced by advanced glycosylation end products(AGEs) in cardiac microvascular endothelial cells.METHODS: Primary cardiac microvascular endothelial cells were cultured in vitro.After treatment with AGEs(100 mg/L) and probucol(5 μmol/L,10 μmol/L,20 μmol/L),NO,iNOS and ROS protein expressions in all groups were detected and compared between groups.RESULTS: Compared with AGEs group,NO and iNOS protein expression increased and ROS protein expression decreased in groups treated with probucol.CONCLUSION: Probucol is able to partially recover AGE-induced dysfunction in cardiac microvascular endothelial cells. |
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| Keywords: | cardiac microvascular endothelial cells advanced glycosylation end products probucol iNOS |
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