|
|||||
|
|
| 大黄素对家兔实验性皮肤创伤的促愈合作用及其机制 | |
| 引用本文： | 唐甜,杨静. 大黄素对家兔实验性皮肤创伤的促愈合作用及其机制[J]. 中国药理学与毒理学杂志, 2006, 20(2): 112-119 |
| 作者姓名： | 唐甜 杨静 |
| 作者单位： | 武汉大学医学院药理学系,湖北,武汉,430071 |
| 摘 要： | 目的探讨大黄素治疗皮肤创伤的可能性及其作用机制。方法采用家兔皮肤切除伤创伤模型,同时滴加绿脓杆菌或金黄色葡萄球菌。大黄素(100~200μg·g-1)敷于创面,每天1次,连续7d或14d。观察创面面积、创面细菌数量和病理组织学改变。生化测定创面组织蛋白和羟脯氨酸(OHP)含量;免疫组化S-P法检测转化生长因子(TGF-β1)含量;逆转录聚合酶链反应测定TGF-β1mRNA表达;Western印迹分析Smad2,3,4和7表达。结果大黄素(100~200μg·g-1)随药物剂量和时间的增加而提高创面愈合百分率和降低感染创面表面细菌数;创面组织蛋白和羟脯氨酸的含量也随着剂量的增加而增加。病理结果显示,大黄素200μg·g-1明显促进创面炎性渗出物吸收、肉芽组织形成和表皮增生。随大黄素浓度的增加,与基质对照组相比,TGF-β1基因和蛋白的表达逐渐增高,均有显著差异;对Smad4蛋白表达无影响,大黄素150和200μg·g-1使Smad7蛋白表达降低;大黄素200μg·g-1使Smad3和Smad2表达增加,其他剂量则无影响。与rhEGF组相比,大黄素100和(或)150μg·g-1组使Smad2和Smad3表达明显下降。结论大黄素促进实验性皮肤创面的修复,其机制可能与TGF-β1和Smads信号转导通路有关。 |
| 关 键 词： | 大黄素 创伤愈合 转化生长因子β 蛋白,Smad |
| 收稿时间： | 2005-09-09 |
| 修稿时间： | 2005-12-19 |
Promoting action of emodin on experimental wound healing and its mechanism in rabbits |
|
| TANG Tian,YANG Jing. Promoting action of emodin on experimental wound healing and its mechanism in rabbits[J]. Chinese Journal of Pharmacology and Toxicology, 2006, 20(2): 112-119 | |
| Authors: | TANG Tian YANG Jing |
| Affiliation: | TANG Tian, YANG Jing* |
| Abstract: | AIM To investigate the availability of emodin in the treatment of dermal wounds and its mechanism. METHODS Full-thickness excision wounds and dripped with Staphylococcus aureus or Pseudomonas pyocyanea were made on the back of rabbits and the emodin was applied to the wound once daily for 7 d or 14 d consecutively and the effect of drug was measured by wound area, bacteria count and histopathological examinations. The contents of hydroxyproline and protein in wound tissues were also measured. Semi-quantitative RT-PCR, Western blotting and immunohistochemistry were used to detect the expression of transforming growth factor-β1 (TGF-β1) and Smad 2, 3, 4 and 7 protein in wound respectively. RESULTS Emodin（100, 150 and 200 μg·g-1） improved wound healing rate and inhibited bacterial growth at the trauma in a concentration and time-dependent manner, total protein and total collagen content of granulation tissues increased with increasing emodin dose too. In addition, that emodin 200 μg·g-1 promoted formation of granulation tissue and rates of epithelialization also confirmed by histopathological examinations. Also, TGF-β1 mRNA and Smad 2 and 3 proteins expression was both up-regulated by emodin in a concentration-dependent manner. On the contrary, there was no significant change in Smad 4 between emodin and vehicle control groups. Emodin 150 and 200 μg·g-1 decreased Smad 7 protein expression and emodin 200 μg·g-1 increased Smad 2 and 3 protein expression,while similar changed didn′t found in other dose. Emodin 150 and 200 μg·g-1 decreased Smad 2 and 3 proteins expression compared with rhEGF group. CONCLUSION Emodin can accelerate healing of cutaneous wounds which is related to TGF-β1/Smads signaling pathway. |
| Keywords: | emodin wound healing transforming growth factor beta proteins Smads |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《中国药理学与毒理学杂志》浏览原始摘要信息 | |
| 点击此处可从《中国药理学与毒理学杂志》下载全文 | |