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| 鬼臼毒素二棕榈酰磷脂酰胆碱前体脂质体的研制及特性观察 | |
| 引用本文： | 林中方,曾抗,周再高,李国锋,谢方明,朱晓亮,张三泉. 鬼臼毒素二棕榈酰磷脂酰胆碱前体脂质体的研制及特性观察[J]. 南方医科大学学报, 2004, 24(7): 784-786 |
| 作者姓名： | 林中方 曾抗 周再高 李国锋 谢方明 朱晓亮 张三泉 |
| 作者单位： | 1. 第一军医大学南方医院皮肤科, 广东, 广州, 510515;2. 第一军医大学南方医院药学部, 广东, 广州, 510515 |
| 基金项目： | 广东省重点工业攻关项目(2003C104034)~~ |
| 摘 要： | 目的 研制鬼臼毒素二棕榈酰磷脂酰胆碱(PPT-DPPC)前体脂质体,提高PPT-DPPC脂质体的稳定性。方法 采用冷冻干燥法,选择海藻糖做冻干剂制备PPT-DPPC前体脂质体,并考察PPT-DPPC前体脂质体水合后的形态、粒径分布、包封率和稳定性。结果 PPT-DPPC前体脂质体经水合后在电镜下呈多层多室脂质体,脂质体粒径分布均匀,平均粒径(1.45±0.38)μm,药物包封率为72.3%。分别在4℃、20℃、40℃贮存1、3、6个月,脂质体形态、粒径及包封率均无明显变化。结论 冷冻干燥法制备PPT-DPPC前体脂质体,方法简便易行,制剂粒径分布均匀、包封率高、有良好稳定性。 |
| 关 键 词： | 鬼臼毒素 二棕榈酰磷脂酰胆碱 前体脂质体 海藻糖 冷冻干燥法 |
| 文章编号： | 1000-2588(2004)07-0784-03 |
| 修稿时间： | 2003-11-30 |
Preparation and characterization of podophyllotoxin-dipalmitoylphosphatidylcholine proliposome |
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| LIN Zhong-fang,ZENG Kang,ZHOU Zai-gao,LI Guo-feng,XIE Fang-ming,ZHU Xiao-liang,ZHANG San-quan. Preparation and characterization of podophyllotoxin-dipalmitoylphosphatidylcholine proliposome[J]. Journal of Southern Medical University, 2004, 24(7): 784-786 | |
| Authors: | LIN Zhong-fang ZENG Kang ZHOU Zai-gao LI Guo-feng XIE Fang-ming ZHU Xiao-liang ZHANG San-quan |
| Affiliation: | LIN Zhong-fang~1,ZENG Kang~1,ZHOU Zai-gao~1,LI Guo-feng~2,XIE Fang-ming~1,ZHU Xiao-liang~1,ZHANG San-quan~1 Department of Dermatology~1,Department of Pharmacy~2,Nanfang Hospital,First Military Medical University,Guangzhou 510515,China |
| Abstract: | Objective To prepare podophyllotoxin-dipalmitoylphosphatidylcholine (PPT-DPPC) proliposomes (PPT-DPPC-PL) for improvement of the stability of PPT-DPPC liposome. Methods Freeze-drying method was used to prepare PPT-DPPC-PL, and the particle morphology, size range, encapsulation efficiency and stability of PPT-DPPC liposome were investigated. Results After hydration of PPT-DPPC-PL, PPT-DPPC liposome appeared multivesicular under electron microscope and the particles were distributed homogenously with an average particle size of 1.45±0.38 μm. The encapsulation efficiency of PPT was 72.3%, and alter storage at 4 to 40℃ for Ⅰ to 6 months, the proliposome remained stable. Conclusion The prepared PPT-DPPC-PL particles by freeze-drying method are evenly distributed. The preparation method is relatively simple with higher embedding ratio and better stability. |
| Keywords: | podophyllotoxin dipalmitoylphosphatidylcholine trehalose proliposome freeze-drying method |
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