| 食管鳞癌细胞外泌体中miR-130a对血管新生的作用及其机制 |
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| 引用本文： | 郑森元,刘芳,赫晓磊,张志强. 食管鳞癌细胞外泌体中miR-130a对血管新生的作用及其机制[J]. 中国癌症防治杂志, 2021, 13(1): 50-55. DOI: 10.3969/j.issn.1674-5671.2021.01.09 |
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| 作者姓名： | 郑森元 刘芳 赫晓磊 张志强 |
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| 作者单位： | 新疆医科大学第一附属医院消化病二科；新疆医科大学第五附属医院老年病科 |
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| 基金项目： | 省部共建中亚高发病成因与防治国家重点实验室开放课题（SKL-HIDCA-2018） |
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| 摘 要： | 目的 探讨食管鳞癌细胞外泌体中的miR-130a对血管新生的作用及其机制.方法 提取食管上皮细胞HEEC、食管鳞癌细胞TE-13和Eca-109所分泌的外泌体及转染miR-130a mimic、miR-130a inhibitor及其阴性对照(NC)后的Eca-109细胞外泌体,并与人脐静脉血管内皮细胞HUVEC共孵育...
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| 关 键 词： | 食管鳞癌 外泌体 miR-130a 血管新生 |
Effects of miR-130a on angiogenesis in exosomes of esophageal squamous cell carcinoma cells and its mechanism |
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| ZHENG Senyuan,LIU Fang,HE Xiaolei,ZHANG Zhiqiang. Effects of miR-130a on angiogenesis in exosomes of esophageal squamous cell carcinoma cells and its mechanism[J]. Journal of Chinese Medical Abstracts·Oncology, 2021, 13(1): 50-55. DOI: 10.3969/j.issn.1674-5671.2021.01.09 |
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| Authors: | ZHENG Senyuan LIU Fang HE Xiaolei ZHANG Zhiqiang |
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| Abstract: | Objective To investigate the effect of miR-130a in esophageal squamous cell carcinoma exosomes on angiogenesis and its mechanism. Methods The exosomes secreted by esophageal epithelial cells HEEC, esophageal squamous cell carcinoma cells TE-13 and Eca-109, and transfected miR-130a mimic, miR-130a inhibitor and its negative control(NC) exosomes of Eca-109 cells were extracted, and co-incubated with human umbilical vein endothelial cells HUVEC. The proliferation, migration and tubule formation abilities of HUVEC cells in each group were detected by MTT, Transwell chamber and tubule formation experiments; the expression level of miR-130a was detected by qRT-PCR; the phosphorylation levels of PI3K and AKT as well as the expression levels of PTEN, Ang1 and iNOS were detected by Western blot. Results After HUVEC cells were co-incubated with TE-13 and Eca-109 cell exosomes, the proliferation, migration and tubule formation abilities were enhanced(P<0.01); and the phosphorylation levels of PI3K and AKT and the expression levels of Ang1 and iNOS were up-regulated(P<0.001), while the expression level of PTEN was down-regulated(P<0.001). The qRT-PCR results showed that the expression level of miR-130a in TE-13 and Eca-109 cell exosomes were higher than that in HEEC exosomes(P<0.01). The down-regulation of miR-130a expression in Eca-109 cell exosomes could inhibit the proliferation, migration and tubule formation abilities of HUVEC cells(P＜0.01), while down-regulated the phosphorylation of PI3K and AKT and the expression levels of Ang1 and iNOS (P＜0.001), and up-regulated the expression level of PTEN(P<0.001). Conclusion The miR-130a in the exosomes of esophageal squamous cell carcinoma exosomes may induce tumor angiogenesis by activating the PTEN/PI3K/AKT signaling pathway of vascular endothelial cells. |
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| Keywords: | Esophageal squamous cell carcinoma Exosomes miR-130a Angiogenesis |
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