Fabrication of capillary‐like structures with Pluronic F127® and Kerria lacca resin (shellac) in biocompatible tissue‐engineered constructs |
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| Authors: | Adam Jacoby Kerry A. Morrison Rachel C. Hooper Ope Asanbe Jeremiah Joyce Remco Bleecker Ross H. Weinreb Hector L. Osoria Sushmita Mukherjee Jason A. Spector |
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| Affiliation: | 1. Hansj?rg Wyss Department of Plastic Surgery, New York University Langone Medical Center, New York, USA;2. Laboratory for Bioregenerative Medicine and Surgery, Division of Plastic Surgery, Weill Cornell Medical College, New York, USA;3. Nancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY;4. Department of Biochemistry, Weill Cornell Medical College, New York, USA |
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| Abstract: | The fabrication of large cellular tissue‐engineered constructs is currently limited by an inability to manufacture internal vasculature that can be anastomosed to the host circulatory system. Creation of synthetic tissues with microvascular networks that adequately mimic the size and density of in vivo capillaries remains one of the foremost challenges within tissue engineering, as cells must reside within 200–300 μm of vasculature for long‐term survival. In our previous work, we used a sacrificial microfibre technique whereby Pluronic® F127 fibres were embedded and then sacrificed within a collagen matrix, leaving behind a patent channel, which was subsequently seeded with endothelial and smooth muscle cells, forming a neointima and neomedia. We now have extended our technique and describe two approaches to synthesize a biocompatible tissue‐engineered construct with macro‐inlet and ‐outlet vessels, bridged by a dense network of cellularized microvessels, recapitulating the hierarchical organization of an arteriole, venule and capillary bed, respectively. Copyright © 2016 John Wiley & Sons, Ltd. |
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| Keywords: | tissue engineering regenerative medicine free tissue transfer flaps sacrificial microfiber networks |
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