Bio‐inspired design of a magnetically active trilayered scaffold for cartilage tissue engineering |
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| Authors: | Alain Vella C. Ross Ethier |
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| Affiliation: | 1. Department of Bioengineering, Imperial College London, UK;2. Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA |
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| Abstract: | An important topic in cartilage tissue engineering is the development of biomimetic scaffolds which mimic the depth‐dependent material properties of the native tissue. We describe an advanced trilayered nanocomposite hydrogel (ferrogel) with a gradient in compressive modulus from the top to the bottom layers (p < 0.05) of the construct. Further, the scaffold was able to respond to remote external stimulation, exhibiting an elastic, depth‐dependent strain gradient. When bovine chondrocytes were seeded into the ferrogels and cultured for up to 14 days, there was good cell viability and a biochemical gradient was measured with sulphated glycosaminoglycan increasing with depth from the surface. This novel construct provides tremendous scope for tailoring location‐specific cartilage replacement tissue; by varying the density of magnetic nanoparticles, concentration of base hydrogel and number of cells, physiologically relevant depth‐dependent gradients may be attained. © 2015 The Authors Journal of Tissue Engineering and Regenerative Medicine Published by John Wiley & Sons Ltd. |
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| Keywords: | biomimetic cartilage magnetic nanoparticles depth‐dependent gradient tissue engineering trilayered scaffold |
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