Platelet‐activated serum might have a therapeutic effect on damaged articular cartilage |
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| Authors: | Munetaka Yokoyama Masato Sato Yoshiki Tani Miyuki Yokoyama Mami Kokubo Masayuki Yamato Teruo Okano Joji Mochida |
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| Affiliation: | 1. Department of Orthopaedic Surgery, Surgical Science, Tokai University School of Medicine, Isehara, Kanagawa, Japan;2. Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan |
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| Abstract: | Platelet‐activated serum (PAS) was collected from rabbits. This contains high concentrations of growth factors, including vascular endothelial growth factor (VEGF), platelet‐derived growth factor (PDGF)‐BB, and transforming growth factor‐beta (TGF‐β). PAS was injected into the knee joints of Japanese White rabbits subjected to anterior cruciate ligament transection (ACL‐T) to investigate its therapeutic effects on articular cartilage. The effect of Avastin (an anti‐VEGF monoclonal antibody) on VEGF expression was also investigated. The levels of VEGF, PDGF‐BB, and TGF‐β in PAS, platelet‐rich plasma (PRP) and autologous serum from untreated rabbits were analysed by enzyme‐linked immunosorbent assays. The samples (n = 24 rabbits) were divided into control (C), PAS (S), Avastin (A) and PAS + Avastin (S + A) treatment groups. Intra‐articular injections were administered weekly for 7 weeks after ACL‐T, during which the weight distribution ratios of the damaged limbs were evaluated. Histological evaluation was performed 12 weeks after ACL‐T using Mankin score. The VEGF, PDGF‐BB and TGF‐β expression levels were significantly higher (P < 0.05) in the PAS than in the PRP or autologous serum samples. The weight distribution ratios of damaged limbs improved significantly after ACL‐T in all treatment groups (P < 0.05). The proximal medial, distal medial and lateral aspects of joints in the treatment groups showed significant differences in Mankin scores compared with controls (P < 0.05). The damaged limb weight distribution ratios, Mankin scores and articular cartilage structure did not differ significantly among the three treatment groups, which all showed significant improvements in structure compared with controls. Copyright © 2017 John Wiley & Sons, Ltd. |
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| Keywords: | platelet‐activated serum articular cartilage repair Avastin vascular endothelial growth factor anterior cruciate ligament transection surgical model |
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