|
|||||
|
|
| 瞬时感受电位M8在人类膝关节软骨组织中的表达 | |
| 引用本文： | 喻 斌,聂博渊,杨朝晖. 瞬时感受电位M8在人类膝关节软骨组织中的表达[J]. 中国组织工程研究, 2014, 18(20): 3117-3121. DOI: 10.3969/j.issn.2095-4344.2014.20.001 |
| 作者姓名： | 喻 斌 聂博渊 杨朝晖 |
| 作者单位： | 山西医科大学第二医院骨科，骨与软组织损伤修复山西省重点实验室，山西省太原市 030001 |
| 基金项目： | 山西省留学人员科研资助项目(2013-114) |
| 摘 要： | 背景：瞬时感受电位通道在人体遍布多种组织和细胞，并参与人体几乎所有的生理功能和许多的病理变化。但是到目前为止，瞬时感受电位M8在人类软骨组织中的表达未见报道。目的：观察瞬时感受电位基因亚家族成员瞬时感受电位M8在人类软骨组织中的表达。方法：15例膝关节软骨样本来源于严重创伤截肢的患者。每个样本取300 mg软骨组织用于组织总蛋白的提取，再取相同部位的软骨组织做切片。Western blot方法检测瞬时感受电位M8在人类软骨组织中的表达，免疫荧光法检测瞬时感受电位M8在人类软骨组织中的分布。结果与结论：Western blot结果显示在相对分子质量为130 000时出现特异性的瞬时感受电位M8条带，并应用Westen印迹技术分析了温度敏感性瞬时感受电位M8通道的年龄相关性，发现随着年龄的增加，其表达量呈下降趋势。免疫荧光染色结果显示瞬时感受电位M8蛋白均匀的分布在细胞膜上。提示瞬时感受电位M8在人类软骨组织中有表达，具体表达在软骨细胞膜上。中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程全文链接： |
| 关 键 词： | 组织构建 软骨组织工程 瞬时感受电位 软骨组织 膝关节 Western blot 免疫荧光染色 |
| 收稿时间： | 2014-04-02 |
Expression of transient receptor potential M8 in human knee joint cartilage |
|
| Yu Bin,Nie Bo-yuan,Yang Zhao-hui. Expression of transient receptor potential M8 in human knee joint cartilage[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(20): 3117-3121. DOI: 10.3969/j.issn.2095-4344.2014.20.001 | |
| Authors: | Yu Bin Nie Bo-yuan Yang Zhao-hui |
| Affiliation: | Department of Orthopedics, Shanxi Provincial Key Laboratory of Bone and Soft Tissue Injury and Repair, Second Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China |
| Abstract: | BACKGROUND: Transient receptor potential protein channel is distributed in many kinds of tissue and cells in human body, and takes part in many physiologic functions and pathological changes of human body. However, up to now, transient receptor potential melastatin 8 (TRPM8) has not been reported in human cartilage.OBJECTIVE: To study the expression of TRPM8, a subfamily member of transient receptor potential gene, in human knee joint cartilage.METHODS: Fifteen samples were selected from patients after amputatioin due to severe trauma. Total protein was extracted from 300 mg cartilage tissue of every patient. To detect the distribution of TRPM8 in human cartilage, cartilage sections were collected in the same region. The expression and distribution of TRPM8 in human cartilage was detected by western blot analysis and immunofluorescence respectively.RESULTS AND CONCLUSION: Western blot analysis showed that specific TRPM8 band was visible at molecular weight of 130 000, and as the age increased, TRPM8 expression in human cartilage was gradually decreased. Immunofluorescence staining results showed that TRPM8 was evenly located in the cell membrane of chondrocytes. TRPM8 is expressed in the human knee cartilage, specifically in member of chondrocytes. |
| Keywords: | transient receptor potential channels cartilage knee joint blotting western immunofluorescence |
| 本文献已被 CNKI 等数据库收录！ | |
| 点击此处可从《中国组织工程研究》浏览原始摘要信息 | |
| 点击此处可从《中国组织工程研究》下载全文 | |