| 自愈水牛血清免疫筛选日本血吸虫cDNA文库 |
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| 引用本文： | 王文琴,刘述先,宋光承,徐裕信,曹建平,陈家旭. 自愈水牛血清免疫筛选日本血吸虫cDNA文库[J]. 中国寄生虫学与寄生虫病杂志, 2003, 21(5): 286-288 |
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| 作者姓名： | 王文琴 刘述先 宋光承 徐裕信 曹建平 陈家旭 |
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| 作者单位： | 中国疾病预防控制中心寄生虫病预防控制所,世界卫生组织疟疾、血吸虫病和丝虫病合作中心,上海,200025 |
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| 摘 要： | 目的 采用自愈水牛血清免疫筛选日本血吸虫成虫cDNA文库并寻找日本血吸虫疫苗新候选抗原。 方法 用感染日本血吸虫后的自愈水牛血清免疫筛选日本血吸虫成虫cDNA文库,将阳性重组子克隆、测序,对核酸序列进行分析,确定目的基因。 结果 从大陆株日本血吸虫成虫λZAPIIcDNA文库筛到26个阳性克隆,经初步鉴定获3种编码蛋白分子的基因:副肌球蛋白,谷胱甘肽S转移酶,羟基类固醇脱氢酶。 结论 感染日本血吸虫后的自愈水牛血清筛选日本血吸虫成虫cDNA文库,可用于寻找日本血吸虫疫苗新候选抗原。
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| 关 键 词： | 日本血吸虫 自愈 cDNA文库 免疫筛选 |
| 文章编号： | 1000-7423(2003)-05-0286-03 |
| 修稿时间： | 2003-07-18 |
Schistosoma japonicum: Screening of cDNA Library with Sera from Self-cured Water Buffaloes(Bos buffelus) |
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| WANG Wen-qin,LIU Shu-xian,SONG Guang-cheng,XU Yu-xin,CAO Jian-ping,CHEN Jia-xu. Schistosoma japonicum: Screening of cDNA Library with Sera from Self-cured Water Buffaloes(Bos buffelus)[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2003, 21(5): 286-288 |
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| Authors: | WANG Wen-qin LIU Shu-xian SONG Guang-cheng XU Yu-xin CAO Jian-ping CHEN Jia-xu |
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| Affiliation: | Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025. |
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| Abstract: | OBJECTIVE: To understand and identify the molecules related to the self-cure of Schistosoma japonicum infection in water buffaloes. METHODS: The S. japonicum adult worm cDNA library was immunologically screened with the sera of self-cured water buffaloes. The positive clones were identified, cloned, sequenced and analysed with software. RESULTS: Three genes encoding antigens relevant to sera antibodies in water buffaloes were cloned and sequenced. These antigens included paramyosin (Sj97), GST, carbonyl reductase-like 20-beta-hydroxysteroid dehydrogenase(CR/20-beta-HSD). CONCLUSION: Sera from self-cured S. japonicum infected water buffaloes can be used to screen adult worm cDNA library for vaccine development. |
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| Keywords: | Schistosoma japonicum self-cure cDNA library immunoscreening clone |
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