| 内毒素血症所致脑血管痉挛与体液及神经内皮素-1表达的关系 |
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| 引用本文： | 万丽丹,林雪群. 内毒素血症所致脑血管痉挛与体液及神经内皮素-1表达的关系[J]. 中国临床解剖学杂志, 2010, 28(5): 562 |
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| 作者姓名： | 万丽丹 林雪群 |
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| 作者单位： | 南昌大学医学院人体解剖学教研室, 南昌 330006 |
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| 摘 要： | 目的探讨内毒素血症大鼠内皮素-1与脑血管痉挛发生与发展的关系。方法 96只Wistar大鼠随机分为对照组、内毒素血症组(注射内毒素第3、6、12、24、48h),以放射免疫检测血浆内皮素-1水平的变化,免疫组织化学ABC法对大鼠大脑中动脉内皮素-1能神经纤维进行染色观察。结果内毒素血症后3、6、12h大鼠血浆内皮素-1水平较对照组明显升高(P0.05),内毒素血症后24和48h已趋于正常(P0.05)。对照组和内毒素血症大鼠大脑中动脉可见棕褐色、呈细线状内皮素-1能免疫反应阳性纤维,以内毒素血症后第6、12h大鼠大脑中动脉内皮素-1免疫反应最为显著(P0.05)。结论内毒素血症后,大鼠内皮素-1合成和释放入血水平升高,脑血管神经纤维内皮素-1的表达水平上调,这两种因素可能同时参与了脑血管痉挛的发生与发展。
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| 关 键 词： | 内毒素血症 脑血管痉挛 大脑中动脉 内皮素-1 |
| 收稿时间： | 2009-10-28 |
The expression and distribution of endothelin-1 in middle cerebral arteries of endotoxemia rats |
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| WAN Li-dan,LIN Xue-qun. The expression and distribution of endothelin-1 in middle cerebral arteries of endotoxemia rats[J]. Chinese Journal of Clinical Anatomy, 2010, 28(5): 562 |
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| Authors: | WAN Li-dan LIN Xue-qun |
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| Affiliation: | Department of Anatomy, Medical School of Nanchang University, Nanchang 330006, China |
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| Abstract: | Objective To investigate the relationship between endothelin-1(ET-1) and cerebral vasospasm in endotoxemia rats. Methods 96 Wistar rats were randomly divided into control and experimental groups, the latter was then subdivided into 3, 6, 12, 24 and 48-h groups after the injection of lipopolysaccharide (LPS). The alteration of plasma ET-1 was detected by radioimmunoassay technique, and ET-1 immunoreactive nerve fibers in middle cerebral artery were examined with immunohistochemistry and measured with stereoscopy method. Results LPS treatment caused a significant increase of plasma ET-1 concentration at 3, 6 and 12 h after the treatment compared to that of control (P<0.05). For 24 and 48-h LPS-treated groups, the plasma ET-1 decreased and closed to the normal level (P>0.05). The ET-1 immunoreactive nerve fibers were observed in all groups, which were brown and thin line-shaped. Moreover, at 6 and 12 h after LPS treatment, the density of ET-1 immunoreactive nerve fibers on middle cerebral arteries was much higher than that of the controls (P<0.05). Conclusions Our findings suggest that, after endotoxemia, the elevated plasma ET-1 concentration, as well enhanced ET-1 immunoreactivity in cerebral arterial nerve fibers, are both involved in the development of cerebral vasospasm induced by endotoxemia. |
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| Keywords: | Endotoxemia Cerebral vasospasm Middle cerebral artery Endothelin-1 |
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