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| 葡萄糖转运蛋白-1在糖尿病早期大鼠视网膜的表达 | |
| 引用本文： | 卜立敏,陈晓隆,杨积文,杨宏伟. 葡萄糖转运蛋白-1在糖尿病早期大鼠视网膜的表达[J]. 眼科新进展, 2015, 0(2): 132-135. DOI: 10.13389/j.cnki.rao.2015.0035 |
| 作者姓名： | 卜立敏 陈晓隆 杨积文 杨宏伟 |
| 作者单位： | 110003 辽宁省沈阳市，沈阳爱尔眼科医院 |
| 摘 要： | 目的 通过检测糖尿病早期大鼠视网膜葡萄糖转运蛋白-１（ｇｌｕｃｏｓｅｔｒａｎｓｐｏｒｔｅｒ-１，ＧＬＵＴ１）表达的变化，揭示ＧＬＵＴ１对早期糖尿病视网膜的影响。方法 选取鼠龄为１２周的健康雄性Ｗｉｓｔａｒ大鼠８０只，随机选取４０只采用链脲佐菌素左下腹腔一次性注射建立１型糖尿病模型（实验中４只因严重感染而死），另取４０只作为对照组注射同等量柠檬酸缓冲液。模型组大鼠建模成功后分别在４周、８周、１２周、１６周（每周９只大鼠）测定血糖和糖化血红蛋白，取同时期对照组（每周１０只）大鼠做相同检测。免疫组织化学方法检测视网膜ＧＬＵＴ１蛋白表达，利用ＡＤＰ酶组织化学染色方法进行视网膜铺片，了解视网膜血管改变情况。结果 糖尿病组血糖及糖化血红蛋白显著高于对照组。免疫组织化学染色、光镜形态学观察显示糖尿病组１６周视网膜节细胞层细胞排列极其紊乱，但未见出血，视网膜内核层水肿，内外核层细胞排列紊乱。糖尿病组ＧＬＵＴ１表达较对照组大鼠视网膜中阳性染色颗粒均明显增加。糖尿病组视网膜ＧＬＵＴ１表达的平均积分光密度在４周（６３．９０３３±４２．２４５３）、８周（９６．３６９１±１８．６９７２）、１２周（１０２．００２８±２３．９９８９）、１６周（１３０．４０８７±７２．７４５１）依次有所增强，其中８周和１２周差异无统计学意义，其他各时间点均有统计学意义（均为Ｐ＜０．０５）；两组各个时间点相比较，差异均有统计学意义（均为Ｐ＜０．０５）。ＡＤＰ酶组织化学染色显示:对照组视网膜血管自视盘发出的大血管向四周呈放射状分布，毛细血管分布均匀；糖尿病组大鼠１６周视网膜出现视盘周围血管灌注降低，周边部部分毛细血管迂曲及血管闭塞。结论 糖尿病大鼠视网膜中ＧＬＵＴ１表达增强，并随病程进展逐渐增高。出现明显视网膜微血管改变时，显著增高。 |
| 关 键 词： | 糖尿病视网膜病变 葡萄糖转运蛋白-1 免疫组织化学 ADP酶组织化学 |
Expression of glucose transporter-l in retina of rats with early-period diabetes |
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| BU Li-Min;CHEN Xiao-Long;YANG Ji-Wen;YANG Hong-Wei. Expression of glucose transporter-l in retina of rats with early-period diabetes[J]. Recent Advances in Ophthalmology, 2015, 0(2): 132-135. DOI: 10.13389/j.cnki.rao.2015.0035 | |
| Authors: | BU Li-Min CHEN Xiao-Long YANG Ji-Wen YANG Hong-Wei |
| Affiliation: | Shenyang Aier Ophthalmologic Hospital. Shenyang 110003 , Liaoning Province . China |
| Abstract: | Objective To investigate the expression of glucose transporter-l ( GLUTI ) in retina of rats with early-period diabetes, and explore the influence of GLUTI on retina of early-period diabetes. Methods A total of 80 healthy male Wistar rats with 12 weeks old were used. Diabetic experiment model in 40 rats were induced by streptozotocin (4 cases died for server infection) , which were injected into the left abdomen . and the other 40 rats were injected tales doses citrate buffer solution as control group. The blood glucose and glycosylated hemoglobin of rats in two groups were examined at 4 weeks .8 weeks , 12 weeks and 16 weeks ,9 cases in each time point of diabetic group and 10 cases of control group. The immunohistochemistry method was used to measure the expression of GLUTI in the retina. ADP enzymohistochemistry stain method of retinal stretched preparation was used to observe the change of retinal blood vessels. Results Blood glucose and glycosylated hemoglobin in diabetic group were significantly higher than those in control group. Immunohistochemical staining and light microscopic morphology showed that the cells in retinal ganglion cell layer arranged in great disorder at 16 weeks in diabetic group with no bleeding ,retinal inner nuclear layer was edema.inner and outer nuclear layer cells arranged in disorder. The positive staining particles of GLUTI expression in the diabetic group was more than that in the control group , there were statistical differences at each time points between two groups ( all P |
| Keywords: | diabetic retinopathy glucose transporter-l immunohistochemistry ADP enzymohistochemistry |
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