| 大肠癌细胞HCT-15肿瘤干细胞样细胞分离及成瘤性比较 |
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| 引用本文： | 邢亚楠,邓 鹏,徐惠绵. 大肠癌细胞HCT-15肿瘤干细胞样细胞分离及成瘤性比较[J]. 现代肿瘤医学, 2015, 0(22): 3226-3228. DOI: 10.3969/j.issn.1672-4992.2015.22.005 |
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| 作者姓名： | 邢亚楠 邓 鹏 徐惠绵 |
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| 作者单位： | 中国医科大学附属第一医院肿瘤外科，辽宁 沈阳 110001 |
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| 基金项目： | 辽宁省科技计划项目(编号:2011225019) |
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| 摘 要： | 目的:依据人大肠癌细胞HCT-15的表面标志物，分离其中的干细胞亚群。建立裸鼠体内原代大肠癌荷瘤模型，比较各亚群肿瘤体积和质量。方法:利用免疫磁珠分选技术，分离其中CD133/CD44干细胞亚群。分选得到CD133+CD44+、CD133+CD44-亚群分别接种于裸鼠体内，并观察肿瘤大小和质量。结果:CD133+CD44+和CD133+CD44-细胞亚群成功的从HCT-15细胞中分离出来。接种CD133+CD44+细胞的裸鼠形成的肿瘤体积［（2.76±0.22）cm3］和质量［（5.2±0.21）g］明显高于接种CD133+CD44-细胞裸鼠的肿瘤体积与质量［（1.56±0.34）cm3，（3.4±0.18）g］（P＜0.05）。结论:CD44阳性的大肠癌肿瘤干细胞成瘤能力明显高于CD44阴性的大肠癌肿瘤干细胞。
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| 关 键 词： | 大肠癌 CD133 CD44 成瘤性 肿瘤干细胞 |
Isolation of cancer stem like cells from colorectal cancer cell HCT -15 and tumorigenicity comparison |
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| Xing Yanan,Deng Peng,Xu Huimian. Isolation of cancer stem like cells from colorectal cancer cell HCT -15 and tumorigenicity comparison[J]. Journal of Modern Oncology, 2015, 0(22): 3226-3228. DOI: 10.3969/j.issn.1672-4992.2015.22.005 |
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| Authors: | Xing Yanan Deng Peng Xu Huimian |
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| Affiliation: | Department of Surgical Oncology,First Affiliated Hospital of China Medical University,Liaoning Shenyang 110001,China. |
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| Abstract: | Objective:According to the surface markers of HCT-15 cancer cell,to isolate the stem cancer cells.To build colorectal cancer xenografts models and compare the tumor size and weight of SCID hairless congenic mice.Methods:We isolated the stem cancer cells from human colorectal cancer cell HCT-15 by magnetic microbeads.CD133+CD44+ and CD133+CD44- stem cancer cells were subcutaneously inoculated to SCID hairless mice，then compared the tumor size and weight.Results:CD133+CD44+ and CD133+CD44- cancer stem cells were isolated successfully from HCT-15 cells.The tumor size derived from CD133+CD44+ cancer stem cells ［(2.76±0.22）cm3］ was larger than CD133+CD44- ones ［(1.56±0.34）cm3］(P＜0.05).And the weight ［(5.2±0.21）g］ was also higher than negative ones ［(3.4±0.18）g］(P＜0.05).Conclusion:The tumorigenicity of CD44 positive colorectal stem cancer cells was significantly higher than negative ones. |
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| Keywords: | colorectal cancer CD133 CD44 tumorigenicity cancer stem cell |
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