| 葫芦素E对非小细胞肺癌细胞的生长抑制作用 |
| |
| 引用本文： | 杨 艳1,温艳惠1,郭海梅1,于治华2,刘千朔2,唐寅思1,李 嵚3. 葫芦素E对非小细胞肺癌细胞的生长抑制作用[J]. 现代肿瘤医学, 2015, 0(16): 2261-2264. DOI: 10.3969/j.issn.1672-4992.2015.16.05 |
| |
| 作者姓名： | 杨 艳1 温艳惠1 郭海梅1 于治华2 刘千朔2 唐寅思1 李 嵚3 |
| |
| 作者单位： | 1.中国医科大学附属第四医院消化内科；2.神经内科；3.中心实验室，辽宁 沈阳 110032 |
| |
| 基金项目： | 国家自然科学基金资助项目（编号:81001028） |
| |
| 摘 要： | 目的:体外观察葫芦素E（cucurbitacin E，CuE）对非小细胞肺癌细胞系A549增殖的影响及分子作用机制。方法:采用MTT法检测不同浓度 CuE（0、1、10、100以及1000nmol/L）处理A549细胞24、48和72小时后的细胞增殖情况。流式细胞仪检测CuE对A549细胞凋亡的影响。 Western blot法检测被处理细胞中p-STAT3、p-Raf-1、p-MEK1/2、p-ERK1/2、Bcl-2、Fas蛋白表达水平的变化。结果:CuE可显著抑制A549细胞的增殖（P＜0.05），且呈时间和浓度依赖性。流式细胞仪检测显示随着CuE作用浓度的逐渐升高（0、100、200、400nmol/L），A549细胞凋亡率由（4.63±0.70）%分别提高到（6.80±0.10）%、（20.53±0.49）%、（24.57±0.55）%，差异有统计学意义（P均<0.05）。Western blot结果显示CuE处理A549细胞，可降低p-STAT3、p-Raf-1、p-MEK1/2、p-ERK1/2、Bcl-2蛋白表达水平，增强Fas蛋白表达水平，且上述作用随CuE浓度升高而增强。结论:CuE可显著抑制A549细胞的增殖并诱导凋亡，其作用机制与阻断STAT3和Raf/MEK/ERK信号通路，同时激活Fas信号通路有关。
|
| 关 键 词： | 葫芦素E 非小细胞肺癌 细胞增殖 细胞凋亡 |
Growth inhibitory effect of cucurbitacin E on non-small cell lung cancer cells |
| |
| Yang Yan1,Wen Yanhui1,Guo Haimei1,Yu Zhihua2,Liu Qianshuo2,Tang Yinsi1,Li Qin3. Growth inhibitory effect of cucurbitacin E on non-small cell lung cancer cells[J]. Journal of Modern Oncology, 2015, 0(16): 2261-2264. DOI: 10.3969/j.issn.1672-4992.2015.16.05 |
| |
| Authors: | Yang Yan1 Wen Yanhui1 Guo Haimei1 Yu Zhihua2 Liu Qianshuo2 Tang Yinsi1 Li Qin3 |
| |
| Affiliation: | 1.Department of Gastroenterology；2.Department of Neurology；3.Laboratory Center，4th Affiliated Hospital of China Medical University，Liaoning Shenyang 110032，China. |
| |
| Abstract: | Objective:To study the in vitro anti-proliferative effects of cucurbitacin E (CuE) and the underlying molecular mechanisms in human non-small cell lung cancer A549 cell line.Methods:MTT assay was performed to examine the growth inhibitory effects of various concentrations of CuE on A549 cells treated for 24,48 and 72 hours.Apoptotic cell death rate after treatment of A549 cells with CuE(100,200 and 400nmol/L) were detected using flow cytometry.Changes of the protein expression levels of p-STAT3,p-Raf-1,p-MEK1/2,p-ERK1/2,Bcl-2,and Fas were determined by Western blot.Results:CuE significantly inhibited the cell proliferation of A549 cells in both a dose- and time-dependent manner(P＜0.05).With CuE concentration increased,the rate of cell apoptosis of A549 cells was significantly increased from (4.63±0.70)% to ［(6.80±0.10）%,100nmol/L CuE］,［(20.53±0.49）%,200nmol/L CuE］ and ［(24.57±0.55）%,400nmol/L CuE］,respectively (P＜0.05).The protein expression levels of p-STAT3,p-Raf-1,p-MEK1/2,p-ERK1/2,Bcl-2 were significantly down-regulated and Fas protein was up-regulated after CuE treatment(P＜0.05).Conclusion:CuE can significantly inhibit proliferation and induce apoptosis on A549 cells via the inhibition of STAT3 and Raf/MEK/ERK signaling pathways as well as the activation of Fas signaling pathway. |
| |
| Keywords: | cucurbitacin E non-small cell lung cancer cell proliferation cell apoptosis |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《现代肿瘤医学》浏览原始摘要信息 |
|
点击此处可从《现代肿瘤医学》下载免费的PDF全文 |
|
