|
|||||
|
|
Shuttle mutagenesis of Legionella pneumophila: identification of a gene associated with host cell cytopathicity. |
|
| Authors: | J Arroyo M C Hurley M Wolf M S McClain B I Eisenstein N C Engleberg |
| Abstract: | We performed shuttle mutagenesis of Legionella pneumophila. Mutants were screened for reduced cellular infectivity. Approximately 10% of the mutants had decreased cytopathicity. The DNA sequence of one locus was determined; the inferred amino acid sequence revealed homology with transport proteins including Escherichia coli TolC, Bordetella pertussis CyaE, and Alcaligenes eutrophus CzcC and CnrC. |
| Keywords: | |