Identification of new antigens in visceral leishmaniasis by expression cloning and immunoblotting with sera of kala-azar patients from Bihar, India |
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| Authors: | Theinert Stephan M Basu Rajatava Forgber Michael Roy Syamal Sundar Shyam Walden Peter |
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| Affiliation: | Clinical Research Group, Department of Dermatology, Charité University Medicine Berlin, Humboldt University, Germany. |
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| Abstract: | Sera of kala-azar patients from Bihar, India, were used to identify Leishmania donovani antigens encoded by a phage expression library. Ten antigens were identified, five of which have not been described as leishmania antigens before. The antigens specifically react with sera of leishmania-infected patients but not of toxoplasma- or plasmodium-infected patients. |
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