| 回转器模拟失重对SGC-7901细胞增生和周期的影响 |
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| 引用本文： | 朱鸣,吴本俨,聂捷琳,李莹辉. 回转器模拟失重对SGC-7901细胞增生和周期的影响[J]. 世界华人消化杂志, 2005, 13(7): 856-859 |
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| 作者姓名： | 朱鸣 吴本俨 聂捷琳 李莹辉 |
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| 作者单位： | 1. 解放军总医院南楼消化科,北京市,100853
2. 航天医院工程研究所第十三研究室,北京市,100094 |
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| 基金项目： | 总装备部预研基金项目,No．51400020204JB1002~~ |
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| 摘 要： |
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| 关 键 词： | 模拟失重 胃癌 细胞增生 细胞周期 |
| 修稿时间： | 2005-01-31 |
Effects of rotating clinostat simulated weightlessness on cell proliferation and cell cycle of human gastric carcinoma cell line SGC-790t |
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| Ming Zhu,Ben-Yan Wu,Jie-Lin Nie,Ying-Hui Li. Effects of rotating clinostat simulated weightlessness on cell proliferation and cell cycle of human gastric carcinoma cell line SGC-790t[J]. World Chinese Journal of Digestology, 2005, 13(7): 856-859 |
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| Authors: | Ming Zhu Ben-Yan Wu Jie-Lin Nie Ying-Hui Li |
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| Affiliation: | Ming Zhu,Ben-Yan Wu,Department of Gastroenterology,South Building,the General Hospital of Chinese PLA,Beijing 100853,China Jie-Lin Nie,Ying-Hui Li,the 13th laboratory of Institute of Space Medico-Engineering,Beijing 100094,China |
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| Abstract: | AIM: To investigate the effect of simulated weightlessness on cell cycle and proliferation of human gastric carcinoma cell line SGC-7901. METHODS: A rotating clinostat was used to simulate weightlessness. The expression of PCNA was examined by immunohistochemical staining. The changes of cell cycle were examined by flow'cytometry. RESULTS: The expression of PCNA did not change significantly after 12 h, 24 h, and 36 h of simulated weightlessness, but decreased significantly after 48 h and 72 h (P<0.05 and P<0.01, respectively). The cell cycle of 12 h, 24 h, and 36 h groups did not change significantly. However, the cells in G0+G1 phase in 48 h and 72 h groups were increased significantly (P<0.01 and P<0.05, respectively), while the cells in S+G2+M phase were decreased significantly (P<0.01 and P<0.05). CONCLUSION: Simulated weightlessness by a clinostat can prevent the transition of cells from G1 phase to S phase, and thus the proliferation of SGC-7901 cells was inhibited. |
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| Keywords: | Simulated weightlessness Gastric cancer Cell proliferation Cell cycle |
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