| 定量ELISA法检测健康人血浆抗呼吸道合胞病毒IgG抗体的可行性研究 |
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| 引用本文： | 刘菊,陈霄. 定量ELISA法检测健康人血浆抗呼吸道合胞病毒IgG抗体的可行性研究[J]. 国际生物制品学杂志, 2012, 35(2): 70-72. DOI: 10.3760/cma.j.issn.1673-4211.2012.02.004 |
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| 作者姓名： | 刘菊 陈霄 |
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| 作者单位： | 610023 成都生物制品研究所有限责任公司质量运营部 |
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| 摘 要： | 目的 确认定量ELISA法检测健康人血浆抗呼吸道合胞病毒（respiratory syncytial virus，RSV）IgG抗体的可行性。方法 采用定量ELISA法检测健康人血浆，对初检的抗-RSV阳性血浆（抗-RSV效价>11 U/ml）进行复检和中和试验。结果 检测3041份血浆，初检和复检的血浆抗-RSV阳性率分别为5.4%和4.7%。定量ELISA法的特异性和精密性均良好，抗-RSV阳性血浆的有效检出率为87.8%。结论 定量ELISA法可用于健康人血浆抗RSV IgG抗体的大规模筛检。
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| 关 键 词： | 酶联免疫吸附测定 呼吸道合胞病毒 人 抗体 血浆 |
Feasibility study on a quantitative ELISA for detecting IgG antibody against respiratory syncytial virus in healthy human plasma |
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| LIU Ju , CHEN Xiao. Feasibility study on a quantitative ELISA for detecting IgG antibody against respiratory syncytial virus in healthy human plasma[J]. International Journal of Biologicals, 2012, 35(2): 70-72. DOI: 10.3760/cma.j.issn.1673-4211.2012.02.004 |
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| Authors: | LIU Ju CHEN Xiao |
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| Affiliation: | Quality Operation Department, Chengdu Institute of Biological Products Co,. Ltd,. Chengdu 610023, China |
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| Abstract: | Objective To identify feasibility of a quantitative ELISA for detecting IgG antibody against respiratory syncytial virus (RSV) in healthy human plasma. Methods The quantitative ELISA was used to detect anti-RSA IgG in healthy human plasma, and anti-RSV positive plasmas (anti-RSV titer＞11 u/ml) obtained by initial detection were detected again by the quantitative ELISA, and a neutralization assay was carried out meanwhile. Results 3041 plasma samples were detected, and plasma anti-RSV positive rates of initial and repeat detection were 5.4% and 4.7%, respectively. The quantitative ELISA had good specificity and precision. The efficient detection rate of anti-RSV positive plasma was 87.8%. Conclusion The quantitative ELISA can be applied to mass screening of anti-RSV IgG antibody in healthy human plasma. |
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| Keywords: | Enzyme-linked immunosorbent assay Respiratory syncytial virus,human Plasma Antibodies |
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