|
|||||
|
|
| 日本血吸虫成虫生殖系统的激光共聚焦显微镜形态学观察 | |
| 引用本文： | 张薇娜,黄大可,张鹏,任翠平,王晓楠,张云霞,沈际佳. 日本血吸虫成虫生殖系统的激光共聚焦显微镜形态学观察[J]. 中国寄生虫学与寄生虫病杂志, 2008, 26(5): 392-394 |
| 作者姓名： | 张薇娜 黄大可 张鹏 任翠平 王晓楠 张云霞 沈际佳 |
| 作者单位： | 1. 安徽医科大学病原生物学教研窜,合肥,230032 2. 安徽医科大学基础医学院综合实验室,合肥,230032 |
| 基金项目： | 国家自然科学基金，安徽省教育厅自然科学基金重点项目 |
| 摘 要： | BALB/c小鼠经皮感染日本血吸虫尾蚴（40±2条/只）35 d后剖杀，获取成虫，经固定、染色、脱色、脱水、透明和封片等处理制成整装玻片标本，用激光共聚焦显微镜观察。显微镜下日本血吸虫的整体形态以及雄虫生殖系统中的睾丸、贮精囊和生殖孔，雌虫生殖系统中的卵巢、卵黄腺、输卵管、卵黄管、受精囊、卵模、梅氏腺、子宫、雌性生殖孔以及虫卵等形态结构清晰。激光共聚焦显微镜技术可较好地观察日本血吸虫虫体各器官组织和细胞结构。 |
| 关 键 词： | 日本血吸虫 生殖系统 细胞学 激光扫描共焦显微镜 |
Morphological Observation on the Reproductive System of Adult Schistosoma japonicum under Confocal Laser Scanning Microscopy |
|
| ZHANG Wei-na,HUANG Da-ke,ZHANG Peng,REN Cui-ping,WANG Xiao-nan,ZHANG Yun-xia,SHEN Ji-jia. Morphological Observation on the Reproductive System of Adult Schistosoma japonicum under Confocal Laser Scanning Microscopy[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2008, 26(5): 392-394 | |
| Authors: | ZHANG Wei-na HUANG Da-ke ZHANG Peng REN Cui-ping WANG Xiao-nan ZHANG Yun-xia SHEN Ji-jia |
| Affiliation: | ZHANG Wei-na1,HUANG Da-ke2,ZHANG Peng1,REN Cui-ping1,WANG Xiao-nan1,ZHANG Yun-xia1,SHEN Ji-jia1(1 Department of Microbiology , Parasitology,Anhui Medical University,Hefei 230032,China,2 Comprehensive Laboratory,Basic Medical Science of Anhui Medical University,China) |
| Abstract: | BABL/c mice were infected with Schistosoma japonicum cercariae (40±2 per mouse) through abdominal skin. Mice were sacrificed after 35 days to acquire the adult worms which were then fixed，stained，clarified，dehydrated and mounted. The specimens were observed under the confocal laser scanning microscope． The overall morphology of the adult worms was displayed distinctly，especially the testicular lobes，seminal vesicle and genital pore of the male，reproductive system，and the ovary，vitelline glands，oviduct，vitelline duct，seminal receptacle，ootype，mehlis gland，uterus，genital pore and eggs of female reproductive system． The confocal laser scanning microscopy is an alternative method to research organs，tissues and cell structure of schistosome worm. |
| Keywords: | Schistosoma japonicum Reproductive ystem Cytology Confocal laser scanning microscopy |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
| 点击此处可从《中国寄生虫学与寄生虫病杂志》浏览原始摘要信息 | |
| 点击此处可从《中国寄生虫学与寄生虫病杂志》下载全文 | |