| 生长抑素通过抑制内质网应激反应减轻肝纤维化小鼠肝损伤研究 |
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| 引用本文： | 都庆国,陆建文,王建华,龙延滨,薛飞. 生长抑素通过抑制内质网应激反应减轻肝纤维化小鼠肝损伤研究[J]. 实用肝脏病杂志, 2021, 24(2): 172-175. DOI: 10.3969/j.issn.1672-5069.2021.02.006 |
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| 作者姓名： | 都庆国 陆建文 王建华 龙延滨 薛飞 |
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| 作者单位： | 710068 西安市 陕西省人民医院普外二科(都庆国,王建华,龙延滨,薛飞); 西安交通大学先进外科技术与工程研究所(陆建文,薛飞); 第一附属医院肝胆外科(陆建文) |
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| 基金项目： | 陕西省自然科学基础研究计划一般项目(编号:2019JQ-973)。 |
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| 摘 要： | 目的 观察生长抑素(SST)对肝纤维化(LF)小鼠肝组织内质网应激(ERS)反应的影响.方法 将40只野生型小鼠随机分为对照组、模型组、小剂量SST干预组和大剂量SST干预组,给予小鼠腹腔内注射25％CCl4橄榄油混合制剂制备肝纤维化模型,分别给予大、小剂量的SST进行干预.取肝组织行天狼猩红和免疫组化染色,对肝组织行...
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| 关 键 词： | 肝纤维化 生长抑素 内质网应激 肝组织炎症和纤维化 小鼠 |
| 收稿时间： | 2020-07-22 |
Somatostatin alleviates liver injury in mice with liver fibrosis by inhibiting endoplasmic reticulum stress |
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| Du Qingguo,Lu Jianwen,Wang Jianhua,et al. Somatostatin alleviates liver injury in mice with liver fibrosis by inhibiting endoplasmic reticulum stress[J]. Journal of Clinical Hepatology, 2021, 24(2): 172-175. DOI: 10.3969/j.issn.1672-5069.2021.02.006 |
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| Authors: | Du Qingguo Lu Jianwen Wang Jianhua et al |
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| Affiliation: | Second Department of General Surgery, Provincial People's Hospital, Xi’an 710068, Shaanxi Province, China |
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| Abstract: | Objective The aim of this study was to observe the effects of somatostatin(SST)on the inflammatory response and apoptosis in mice with CCl4-induced liver fibrosis(LF),and to investigate whether SST can reduce liver injury and alleviate the process of LF by inhibiting the ERS.Methods Forty WT mice were randomly divided into control,model,low-dose SST-intervened and large-dose SST-intervened group,with 10 in each.Except the mice in control,the mice in other groups were injected with 25%CCl4 and oil mixture intraperitoneally to establish the model of liver injury and the mice in SST-intervened groups were given large or low doses of SST subcutaneously,respectively.Serum ALT and AST were detected,the liver tissues were obtained for pathological examination,and the protein expression of Bcl-2,Bax,Casp-9,BiP and CHOP in liver tissues were detected by Western blot.Results The histological activity index(HAI)score in model group was(12.2±2.7),significantly higher than[(1.8±0.8),P<0.01]in the control,and the hepatic fibrosis score was(5.4±0.5),significantly higher than[(0.1±0.3),P<0.01]in control;serum AST and ALT in the model were(194.7±35.2)U/L and(121.8±26.6)U/L],both significantly higher than[(53.8±21.7)U/L and(35.3±12.9)U/L,respectively,P<0.01]in the control;the HAI scores in low-dose and large-dose SST-intervened groups were(7.7±2.1)and(6.9±2.0),and the hepatic fibrosis scores were(4.3±1.0)and(3.9±0.9),significantly decreased compared to those in the model(P<0.05);the expression of Bax,Casp-9,BiP and CHOP in hepatic tissues in the model intensified greatly compared to in the control([P<0.01),while the hepatic expression of Bax,Casp-9,BiP and CHOP in SST-intervened groups weaken obviously compared to in the model(F=26.36,F=14.81,F=25.11 and F=30.31,P<0.05).Conclusion SST could alleviate liver inflammation and LF,the mechanism by which might be related to the inhibition of ERS. |
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| Keywords: | Liver fibrosis Somatostatin Endoplasmic reticulum stress Histological activity index Mice |
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