| 长链非编码RNA BCYRN1对SKOV3细胞恶性生物学行为的影响 |
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| 引用本文： | 李喜梅,林丽,毛郁蕾,陈新宵,冯泽蛟,廖海红. 长链非编码RNA BCYRN1对SKOV3细胞恶性生物学行为的影响[J]. 中华全科医学, 2021, 19(10): 1658-1661,1728. DOI: 10.16766/j.cnki.issn.1674-4152.002135 |
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| 作者姓名： | 李喜梅 林丽 毛郁蕾 陈新宵 冯泽蛟 廖海红 |
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| 作者单位： | 1.温州市中心医院妇产科,浙江 温州 325000 |
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| 基金项目： | 浙江省医药卫生科技计划项目2019RC285 |
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| 摘 要： | 目的 探讨长链非编码RNA brain cytoplasmic RNA 1在卵巢癌组织中的表达及对SKOV3细胞恶性生物学行为的影响.方法 选取温州市中心医院2017年3月-2019年3月收治的32例卵巢癌患者癌组织及其相应的 癌旁组织标本,正常卵巢上皮细胞系IOSE和卵巢癌细胞系(SKOV3、HO8910、A2780...
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| 关 键 词： | LncRNA brain cytoplasmic RNA 1 卵巢癌 SKOV3细胞株 增殖 转移 |
| 收稿时间： | 2020-12-12 |
Effect of long chain noncoding RNA BCYRN1 on malignant biological behavior of SKOV3 cells |
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| Affiliation: | Department of Obstetrics and Gynecology, Wenzhou Central Hospital, Wenzhou, Zhejiang 325000, China |
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| Abstract: | Objective To investigate the expression of long chain non-coding RNA brain cytoplasmic RNA-1 (BCYRN1) in ovarian cancer and its effect on the malignant biological behaviour of SKOV3 cells. Methods A total of 32 ovarian cancer tissues and their corresponding adjacent tissues treated in Wenzhou Central Hospital from March 2017 to March 2019 were selected. Normal ovarian epithelial cell lines (IOSE) and ovarian cancer cell lines (SKOV3, HO8910, A2780) were purchased from ATCC. The level of BCYRN1 was detected by real-time PCR. SKOV3 cells were transfected with BCYRN1 high-expression plasmid and low-expression plasmid and their corresponding control substances, and cell lines with high and low expression of BCYRN1 were constructed. BrdU assay was used to detect cell proliferation, scratch healing test was used to detect cell migration, and Transwell assay was used to detect cell invasion. Results The expression of BCYRN1 in ovarian cancer tissues (5.64±1.02) was higher than that in adjacent tissues (2.12±0.65), and the levels of BCYRN1 in SKOV3, HO8910 and A27809 cells were (2.25±0.78), (2.85±0.78) and (3.12±1.01), respectively, which were significantly higher than those of IOSE (1.01±0.12). The high expression of BCYRN1 promoted the proliferation, migration and invasion of SKOV3 cells. Conclusion The expression of BCYRN1 is up-regulated in ovarian cancer, and it can be used as an oncogene to promote the progression of ovarian cancer. |
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