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| 罗哌卡因抑制Rac1/JNK1/FAK通路的活化抑制黑色素瘤M21细胞生长、侵袭和迁移 | |
| 引用本文： | 夏 夏1,田国锐1,董海波2,丁志军2,段 伟2. 罗哌卡因抑制Rac1/JNK1/FAK通路的活化抑制黑色素瘤M21细胞生长、侵袭和迁移[J]. 现代肿瘤医学, 2021, 0(14): 2422-2428. DOI: 10.3969/j.issn.1672-4992.2021.14.007 |
| 作者姓名： | 夏 夏1 田国锐1 董海波2 丁志军2 段 伟2 |
| 作者单位： | 1.恩施土家族苗族自治州中心医院麻醉科，湖北 恩施 445000;2.武汉大学附属中南医院麻醉科，湖北 武汉 430071 |
| 基金项目： | 湖北省自然科学基金资助项目（编号:2016CFB168） |
| 摘 要： | 目的:本文旨在探究罗哌卡因(ropivacaine,RPC)对黑色素瘤M21细胞生长，运动和上皮间质形态转换的影响。方法:采用0、0.25、0.5、1 mmol/L剂量罗哌卡因处理M21细胞，将细胞随机分为四组:RPC 0 mmol/L组、RPC 0.25 mmol/L组、RPC 0.5 mmol/L组和RPC 1 mmol/L组进行后续试验。EDU染色检测细胞生长；流式细胞仪检测细胞凋亡率；Transwell检测侵袭细胞数；划痕试验检测细胞迁移；显微镜观察上皮间充质转化形态学变化；蛋白免疫印迹检测E-cadherin、Vimentin、N-cadherin、Rac1、JNK1、p-JNK1、FAK、p-FAK蛋白表达水平。结果:结果表明，与RPC 0 mmol/L组相比较，RPC 0.5、1 mmol/L组EDU阳性细胞数显著降低（P＜0.05），细胞凋亡率显著升高（P＜0.05），侵袭细胞数显著降低（P＜0.05），划痕愈合率显著降低（P＜0.05），上皮间充质转化受到抑制，E-cadherin蛋白表达水平显著升高（P＜0.05），Vimentin、N-cadherin、Rac1蛋白表达水平显著降低（P＜0.05），p-JNK1/JNK1、p-FAK/FAK比值显著降低（P＜0.05）。结论:罗哌卡因抑制Rac1/JNK1/FAK通路的活化调节黑色素瘤M21细胞生长，运动和上皮间质形态转换。 |
| 关 键 词： | 黑色素瘤 罗哌卡因 上皮间充质转化 Rac1/JNK1/FAK通路 |
Ropivacaine inhibits the activation of Rac1/JNK1/FAK pathway to inhibit the growth,invasion and migration of melanoma M21 cells |
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| XIA Xia1,TIAN Guorui1,DONG Haibo2,DING Zhijun2,DUAN Wei2. Ropivacaine inhibits the activation of Rac1/JNK1/FAK pathway to inhibit the growth,invasion and migration of melanoma M21 cells[J]. Journal of Modern Oncology, 2021, 0(14): 2422-2428. DOI: 10.3969/j.issn.1672-4992.2021.14.007 | |
| Authors: | XIA Xia1 TIAN Guorui1 DONG Haibo2 DING Zhijun2 DUAN Wei2 |
| Affiliation: | 1.Anesthesia Department of Enshi Tujia and Miao Autonomous Prefecture Central Hospital,Hubei Enshi 445000,China；2.Department of Anesthesiology,Central South Hospital,Wuhan University,Hubei Wuhan 430071,China. |
| Abstract: | Objective:To investigate the effect of ropivacaine on the growth,movement and morphological transformation of epithelial mesenchymal(M21)cells in melanoma.Methods:The M21 cells were treated with ropivacaine doses of 0,0.25,0.5 and 1 mmol/L,and the cells were randomly divided into four groups:RPC 0 mmol/L group,RPC 0.25 mmol/L group,RPC 0.5 mmol/L group and RPC 1 mmol/L group for subsequent experiments.Cell growth was detected by EDU staining.Apoptosis rate was detected by flow cytometry.Transwell detected the number of cells invaded.Cell migration was detected by scratch test.Morphological changes of epithelial mesenchymal transformation were observed under microscope.Western blot was used to detect the expression of E-cadherin,Vimentin,N-cadherin,Rac1,JNK1,p-JNK1,FAK and p-FAK.Results:Results show that compared with the RPC 0 mmol/L group,RPC 0.5,1 mmol/L group EDU positive cells was significantly reduced（P＜0.05）,the apoptosis rate increased significantly（P＜0.05）,invasive cells was significantly reduced（P＜0.05）.Nick healing rate significantly decreased（P＜0.05）,between epithelial mesenchymal transformation is restrained,E-cadherin protein levels significantly increased（P＜0.05）,Vimentin,N-cadherin protein were significantly reduced（P＜0.05）,Rac1 protein level and p-JNK1/JNK1 and p-FAK/FAK ratio were significantly reduced（P＜0.05）.Conclusion:Ropivacaine inhibits the activation of Rac1/JNK1/FAK pathway and regulates the growth,motility and morphological transformation of melanoma M21 cells. |
| Keywords: | melanoma ropivacaine epithelial mesenchymal transformation Rac1/JNK1/FAK pathway |
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