| 应用优化的全自动免疫组化染色方案检测错配修复蛋白MLH 1、PMS2在结直肠癌组织中的表达 |
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| 引用本文： | 付 萍,林 俐,王秋实,敖 杰,马 强,赵连花,毛成毅,肖华亮. 应用优化的全自动免疫组化染色方案检测错配修复蛋白MLH 1、PMS2在结直肠癌组织中的表达[J]. 现代肿瘤医学, 2021, 0(13): 2254-2257. DOI: 10.3969/j.issn.1672-4992.2021.13.010 |
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| 作者姓名： | 付 萍 林 俐 王秋实 敖 杰 马 强 赵连花 毛成毅 肖华亮 |
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| 作者单位： | 陆军军医大学大坪医院病理科，重庆 400042 |
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| 摘 要： | 目的:通过优化的全自动免疫组化检测方案提高MLH1和PMS2蛋白免疫组化检测的稳定性.方法:收集2018年至2019年大坪医院结直肠癌手术标本20例,经石蜡包埋、切片、染色,显微镜下观察错配修复蛋白表达的差异.采用Ventana公司生产的BenchMark Ultra,二抗检测系统分别采用UltraView Unive...
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| 关 键 词： | 免疫组化 全自动免疫组化染色 错配修复蛋白 结直肠癌 |
Detection of mismatch repair protein MLH1 and PMS2 expression in colorectal cancer tissues by optimized automatic immunohistochemical staining scheme |
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| FU Ping,LIN Li,WANG Qiushi,AO Jie,MA Qiang,ZHAO Lianhua,MAO Chengyi,XIAO Hualiang. Detection of mismatch repair protein MLH1 and PMS2 expression in colorectal cancer tissues by optimized automatic immunohistochemical staining scheme[J]. Journal of Modern Oncology, 2021, 0(13): 2254-2257. DOI: 10.3969/j.issn.1672-4992.2021.13.010 |
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| Authors: | FU Ping LIN Li WANG Qiushi AO Jie MA Qiang ZHAO Lianhua MAO Chengyi XIAO Hualiang |
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| Affiliation: | Department of Pathology,Daping Hospital,Army Medical University,Chongqing 400042,China. |
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| Abstract: | Objective:To improve the stability of immunohistochemical detection of MLH1 and PMS2 proteins by optimized automatic immunohistochemical staining scheme.Methods:Twenty cases of colorectal cancer surgical specimens from Daping Hospital from 2018 to 2019 were collected.The expression of MLH1 and PMS2 was observed by microscope after paraffin embedding,sectioning and staining.UltraView Universal DAB Detection kit (UV) and Optiview DAB IHC Detection kit (OV) and amplification kit were used for the secondary antibody detection system.Results:In the detection of MLH1 and PMS2,signal obtained by UV secondary antibody detection system in BenchMark Ultra dyeing machine was weak,accompanied with blurry background,making the expression of PMS2 appear false negative.After the optimization of the dyeing scheme,OV secondary antibody detection system was used.The detection of MLH1 and PMS2 (PMS2 required amplification kit) staining were better than that of UV secondary antibody detection system.Compared with manual staining,the dyeing intensity and positive rate were slightly better than manual staining.Conclusion:The optimum conditions should be explored and optimized before the application of automatic immunohistochemical staining machine,especially in the detection of MLH1 and PMS2,thus to obtain the best staining scheme for the antibodies. |
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| Keywords: | IHC automatic immunohistochemical staining mismatch repair colorectal cancer |
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