| 金雀异黄酮对大鼠视网膜缺血-再灌注损伤的保护作用 |
| |
| 引用本文： | 张静. 金雀异黄酮对大鼠视网膜缺血-再灌注损伤的保护作用[J]. 眼科新进展, 2021, 0(12): 1122-1126. DOI: 10.13389/j.cnki.rao.2021.0235 |
| |
| 作者姓名： | 张静 |
| |
| 作者单位： | 637000 四川省南充市,川北医学院第二附属医院眼科 |
| |
| 摘 要： | 目的 探讨金雀异黄酮(GEN)对大鼠视网膜缺血-再灌注(L/R)损伤的保护作用.方法 选取30只健康SPF级大鼠,按照随机数字表法将其分为假手术组、I/R组和L/R+ GEN组,每组各10只.I/R组和I/R+ GEN组大鼠采用前房灌注生理盐水升高眼压法制备视网膜I/R损伤大鼠模型,I/R组大鼠术后每天给予注射生理盐水...
|
| 关 键 词： | 金雀异黄酮 缺血-再灌注损伤 氧化应激 |
Protective effect of genistein on retinal ischemia-reperfusion injury in rats |
| |
| ZHANG Jing. Protective effect of genistein on retinal ischemia-reperfusion injury in rats[J]. Recent Advances in Ophthalmology, 2021, 0(12): 1122-1126. DOI: 10.13389/j.cnki.rao.2021.0235 |
| |
| Authors: | ZHANG Jing |
| |
| Affiliation: | Department of Ophthalmology, the Second Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, Sichuan Province, China |
| |
| Abstract: | Objective To explore the protective effect of genistein (GEN) on retinal ischemia-reperfusion (I/R) injury in rats.Methods Thirty healthy SPF rats were selected and randomly divided into the sham operation group, I/R group, and I/R+GEN group, with 10 rats in each group. In the I/R group and I/R+GEN group, I/R rat models were prepared by anterior chamber infusion of normal saline to increase intraocular pressure. In the I/R group, rats were injected with 1 mL·kg-1·d-1 normal saline every day after operation. In the I/R+GEN group, rats were injected with 40 mL·kg-1·d-1 GEN every day after operation. In the sham operation group, rats were injected with 1 mL·kg-1·d-1 normal saline every day after the corresponding sham operation. Seven consecutive days later, all rats were dislocated and sacrificed, and their eye tissues were taken. The hematoxylin and eosin (HE) staining was used to observe the retinal morphology and thickness of inner plexiform layer (IPL), inner nuclear layer (INL) and ganglion cell layer (GCL). The immunofluorescence assay was used to determine the survival rate of retinal ganglion cells (RGC). The TUNEL staining was used to detect the apoptosis of retinal cells. The catalase (CAT), malondialdehyde (MDA), and superoxide dismutase (SOD) were measured to reflect the oxidative stress state of rats’ retinas. Western blot was used to analyze the expression of NLRP3, ASC, and Caspase-1 proteins in the retina of all rats.Results The total retinal thickness of rats in the I/R group, I/R+GEN group, and sham operation group were (114.37±7.32) μm, (155.31±6.83) μm, and (178.98±13.65) μm, respectively. The differences among the three groups were statistically significant (t=14.284, all P<0.01). The thickness of IPL, INL, and GCL of rats were (18.95±5.06) μm, (17.62±4.69) μm, and (19.03±4.74) μm in the I/R group, (20.69±8.13) μm, (25.74±6.78) μm, and (26.71±7.85) μm in the I/R+GEN group, (11.73±3.15) μm, (11.97±3.56) μm, and (12.59±3.24) μm in the sham operation group (all P<0.05). The differences among the three groups were statistically significant (all P<0.01). The survival rates of RGCs in the retina of rats in the I/R group, I/R+GEN group, and sham operation group were (26.87±3.12) %, (73.46±7.80) %, and (100.00±5.64) %, respectively. The differences among the three groups were statistically significant (t=16.825, all P<0.01). The content of CAT, MDA, and SOD in the retina of rats showed statistically significant differences among the three groups (all P<0.01). In the I/R group, the three indexes were statistically different from those in the I/R+GEN group and the sham operation group (all P<0.05). Western blot results showed that there were statistically significant differences in the expression levels of NLRP3, ASC, and Caspase-1 in the retina of rats among the three groups (all P<0.01), and these indexes also showed statistically significant differences between any two groups (all P<0.05).Conclusion GEN can improve the anti-inflammatory and antioxidant capacity of retinal cells to inhibit apoptosis, thus enhancing the protective effect on retinal I/R injury in rats. |
| |
| Keywords: | genistein ischemia-reperfusion injury oxidative stress |
|
| 点击此处可从《眼科新进展》浏览原始摘要信息 |
|
点击此处可从《眼科新进展》下载免费的PDF全文 |
|
