| 阴道毛滴虫低温保存及其影响因素 |
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| 引用本文： | 袁玉青,薛长贵. 阴道毛滴虫低温保存及其影响因素[J]. 中国寄生虫学与寄生虫病杂志, 2010, 28(5): 395-396 |
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| 作者姓名： | 袁玉青 薛长贵 |
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| 作者单位： | 郑州大学基础医学院寄生虫学教研室,郑州 450001 |
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| 摘 要： | 观察不同浓度二甲亚砜(DMSO)和甘油、不同密度滴虫及不同冻存时间对阴道毛滴虫在-78℃低温保存的影响。阴道毛滴虫的最适冻存密度为(1～2)×106/ml。10%甘油和10%二甲亚砜的冻存效果最好,复苏存活率分别为38.0%和31.7%,两者差异无统计学意义(P0.05)。短期冻存(1～16周)的效果良好,均可在37℃重悬培养至对数生长期。
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| 关 键 词： | 阴道毛滴虫 低温保存 二甲亚砜 甘油 |
Cryopreservation of Trichomonas vaginalis and its influence factors |
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| YUAN Yu-qing,XUE Chang-gui. Cryopreservation of Trichomonas vaginalis and its influence factors[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2010, 28(5): 395-396 |
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| Authors: | YUAN Yu-qing XUE Chang-gui |
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| Affiliation: | Department of Parasitology,Basic Medical College of Zhengzhou University,Zhengzhou 450001,China |
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| Abstract: | Different concentrations of dimethylsulfoxide （DMSO） and glycerol, various densities of Trichomonas vaginalis trophozoites and duration of storage were used to study the effects on the cryopreservation of T. vaginalis at -78 ℃. The optimal densities of trichomonads for cryopreservation were （1-2） ×106/ml. Glycerol and DMSO showed a high protective effect, with a maximum effect at a concentration of 10%, and the survival rates were 38.0% and 31.7%, respectively. There was no significant difference between them（P＞0.05）. The results showed that the above optimized factors can provide a good protection in short-term （1-16 weeks） cryopreservation, and the trichomonads can be incubated to a logarithmic growth phase at 37 ℃. |
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| Keywords: | Trichomonas vaginalis Cryopreservation Dimethylsulfoxide Glycerol ')" >Trichomonas vaginalis Cryopreservation Dimethylsulfoxide Glycerol |
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