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Sensitive and specific detection of Yersinia pseudotuberculosis by loop-mediated isothermal amplification |
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| Authors: | Horisaka Tomoko Fujita Kayoko Iwata Taketoshi Nakadai Aya Okatani Alexandre T Horikita Tetsuya Taniguchi Takahide Honda Eiichi Yokomizo Yuichi Hayashidani Hideki |
| Affiliation: | Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan. |
| Abstract: | We developed a loop-mediated isothermal amplification method able to detect Yersinia pseudotuberculosis strains in 30 min by using six primers designed by targeting the inv gene. This method is more sensitive than PCR and might be a useful tool for detecting and identifying Y. pseudotuberculosis. |
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